Adenyl cyclase. II. The enzymatically catalyzed formation of adenosine 3',5'-phosphate and inorganic pyrophosphate from adenosine triphosphate.
Adenyl cyclase. II. The enzymatically catalyzed formation of adenosine 3',5'-phosphate and inorganic pyrophosphate from adenosine triphosphate.
复制标题
腺苷酸环化酶。
DOI：
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发表时间：
1962
影响因子：
4.8
通讯作者：
E. Sutherland
中科院分区：
文献类型：
--
作者：
T. Rall;E. Sutherland
SUMMARY 1. Adenosine 3’) 5’-phosphate-Pz2 (cyclic 3’) 5’-AMP32) was formed by adenyl cyclase in the presence of AP32PP with a specific activity of 67 to 84% of the precursor. Little radio- active cyclic 3’) 5’-AMP was formed in the presence of SPP32P32. 2. When solubilized preparations of adenyl cyclase skele- tal muscle and cerebral cortex were employed, 0.8 to 2.5 pmoles of inorganic pyrophosphate per pmole of cyclic 3’,5’-AMP were formed. The accumulation of both products was inhibited by Zn++ ions. 3, When particulate preparations of adenyl cyclase from cardiac muscle were used, 1.5 to 2.1 pmoles of inorganic pyro- phosphate per pmole of cyclic 3’,5’-AMP were formed. The accumulation of both products was increased by epinephrine. 4. It was observed that the possible formation of other nucleo- side 3’) 5’-phosphates from the corresponding nucleoside triphos- phates by cyclase preparations could not be readily detected with existing methods. Inosine 3’,5’-phosphate had the great- est activity the assay system, which amounted to only ap-
