Role of spleen-derived monocytes/macrophages in acute ischemic brain injury
Role of spleen-derived monocytes/macrophages in acute ischemic brain injury
复制标题
脾源性单核细胞/巨噬细胞在急性缺血性脑损伤中的作用
DOI：
10.1038/jcbfm.2014.101
复制
发表时间：
2014-08-01
影响因子：
6.3
通讯作者：
Cho, Sunghee
中科院分区：
文献类型：
--
作者：
Kim, Eunhee;Yang, Jiwon;Cho, Sunghee
单核细胞/巨噬细胞（MMs），即单核吞噬细胞，已被认为与中风诱导的炎症和损伤有关。然而，促炎性Ly - 6C（高）和抗炎性Ly - 6C（低）单核细胞亚群的存在，使得它们在中风病理评估中的作用存在不确定性。随着近期发现脾脏是MMs的即时储存库，本研究探讨了脾脏来源的MMs是否在中风病理评估中是必需的。我们观察到中风后的动物脾脏收缩，并且这种收缩伴随着脾脏中Ly - 6C（高）和Ly - 6C（低）亚群数量的减少。这些亚群从脾脏的迁移在时间上与缺血大脑中相应的增加相吻合。与有脾脏的小鼠相比，在中风前刚刚接受脾切除的小鼠大脑中Ly - 6C（高）和Ly - 6C（低）MMs的积聚较少。尽管这两种亚群的积聚减少，但无脾小鼠的梗死面积和肿胀并没有减少。缺血后脑梗死面积和MM浸润程度的分离结果表明，脾脏来源的总MMs在急性梗死发展中的作用极小。建议选择性地针对Ly - 6C（高）或Ly - 6C（低）MM，以明确各个亚群对急性中风病理评估的贡献。
Monocytes/macrophages (MMs), mononuclear phagocytes, have been implicated in stroke-induced inflammation and injury. However, the presence of pro-inflammatory Ly-6C(high) and antiinflammatory Ly-6C(low) monocyte subsets raises uncertainty regarding their role in stroke pathologic assessment. With recent identification of the spleen as an immediate reservoir of MMs, this current study addresses whether the spleen-derived MMs are required for stroke pathologic assessment. We observed that the spleen was contracted in poststroke animals and the contraction was accompanied by decreased number of Ly-6Chigh and Ly-6C(low) subsets in the spleen. The deployment of these subsets from the spleen temporally coincided with respective increases in the ischemic brain. Compared to mice with the spleen, mice receiving a splenectomy just before the stroke displayed less accumulation of Ly-6C(high) and Ly-6C(low) MMs in the brain. Despite the reduced accumulation of both subsets, infarct size and swelling were not reduced in the asplenic mice. The dissociative findings of infarct size and extent of MM infiltration in the postischemic brain indicate minimal involvement of spleen-derived total MMs in acute infarct development. Selective Ly-6C(high) or Ly-6C(low) MM targeting is suggested to address the contribution of the individual subset to acute stroke pathologic assessment.