Crystallization of nucleic acids and proteins
Crystallization of nucleic acids and proteins
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DOI：
10.1093/oso/9780199636792.001.0001
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
A. Ducruix;R. Giegé
中科院分区：
文献类型：
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作者：
A. Ducruix;R. Giegé
晶体学是测定生物大分子结构的主要方法，但结晶技术仍然被认为是难以实现的。这个新的版本的核酸和蛋白质的结晶：一个实用的方法继续在第一版的静脉提供了一个详细的和合理的指导，以生产足够数量和质量的蛋白质和核酸晶体的衍射研究。它已经彻底更新，包括所有主要的新技术，如分子生物学在结构生物学中的应用（最大化表达系统，序列修改以实现结晶，以及引入异常散射体）；预成核和成核的光谱诊断分析以及平面脂质膜上可溶性蛋白的二维电子晶体学。除了对晶体发生的介绍外，其他主题还包括：处理大分子溶液，实验设计，播种，从溶液到晶体的处理凝胶结晶核酸复合物结晶和膜蛋白浸泡技术晶体的初步表征，以确定它们是否适合衍射研究。与所有实用方法书籍一样，这些协议都是由经验丰富的研究人员编写的，并且是经过测试的方法。基础理论与实验室协议结合在一起，为研究人员提供了开发这些强大技术所需的概念和方法工具。《核酸和蛋白质的结晶：实用方法2》将是分子生物学、晶体学、蛋白质工程和生物化学研究人员实用结晶方法的宝贵手册。
Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two-dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucliec Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.