Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum.
Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum.
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中药一贯健含药血清增强离体基质细胞衍生因子1介导的小鼠骨髓间充质干细胞向肝细胞的分化
DOI：
10.1155/2016/7380439
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Fu L;Pang B;Zhu Y;Wang L;Leng A;Chen H
益官健是治疗肝脏疾病的中药，已被证明可以减少肝纤维化。本实验研究了益冠健含药血清（YGJ）与基质细胞衍生因子1 （SDF-1）和肝细胞生长因子（HGF）对小鼠骨髓间充质细胞（BM-MSCs）向肝细胞分化的影响。从小鼠骨髓中分离出粘附的MSCs。20 ng/mL HGF、50 ng/mL SDF-1和20%含一冠健药物血清诱导分化7 ~ 28 d，采用免疫细胞化学和western blot检测成熟肝细胞标志物白蛋白（ALB）和胆管细胞标志物角蛋白-18 （CK-18）。传代培养后BM-MSCs呈均匀的纺锤形生长，CD90染色阳性，CD34染色阴性。HGF +正常血清或YGJ诱导14天，HGF + SDF-1 +正常血清诱导7天，HGF + SDF-1 + YGJ诱导5天后，MSCs形态逐渐改变，开始类似肝细胞样细胞。在第7天，与HGF + SDF-1 +正常血清相比，添加HGF + SDF-1 + YGJ培养物的ALB和CK-18阳性细胞比例显著高于添加HGF + SDF-1 +正常血清的培养物。这些观察结果证实了western blot的结果。综上所述，益冠健含药血清能促进小鼠BM-MSCs体外向肝细胞分化，并与SDF-1、HGF具有协同作用。
Yiguanjian is administered in traditional Chinese medicine for liver diseases and has been demonstrated to reduce liver fibrosis. This study investigated the effect of Yiguanjian drug-containing serum (YGJ) with Stromal Cell-Derived Factor 1 (SDF-1) and Hepatocyte Growth Factor (HGF) on the differentiation of murine bone-marrow-derived mesenchymal cells (BM-MSCs) into hepatocytes in vitro. Adherent MSCs were isolated from murine bone marrow. Differentiation was induced by 20 ng/mL HGF, 50 ng/mL SDF-1, and 20% Yiguanjian drug-containing serum for 7 to 28 days, and mature hepatocytes' marker albumin (ALB) and cholangiocytes' marker cytokeratin-18 (CK-18) were assessed by immunocytochemistry and western blot. BM-MSCs exhibited homogeneous spindle shape growth after subculture and stained positive for CD90 and negative for CD34. After induction with HGF + normal serum or YGJ for 14 days, HGF + SDF-1 + normal serum for 7 days, or HGF + SDF-1 + YGJ for 5 days, MSCs' morphology changed gradually and begun to resemble hepatocyte-like cells. Cultures supplemented with HGF + SDF-1 + YGJ contained significantly higher proportions of ALB and CK-18 positive cells than cultures supplemented with HGF + SDF-1 + normal serum at day 7. These observations corroborated the results of western blot. In conclusion, Yiguanjian drug-containing serum could facilitate the differentiation of murine BM-MSCs into hepatocytes in vitro and has a synergistic effect with SDF-1 and HGF.