Fine‐scale population fragmentation of a grassland butterfly Plebejus argyrognomon inhabiting agricultural field margin and riverbank in rural landscapes
Fine‐scale population fragmentation of a grassland butterfly Plebejus argyrognomon inhabiting agricultural field margin and riverbank in rural landscapes
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DOI：
10.1111/ens.12491
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发表时间：
2021-09
影响因子：
0.9
通讯作者：
T. Miyashita;Fuga Matsui;Hidenori Deto;Tatsuya Imai;N. Kondo
中科院分区：
文献类型：
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作者：
T. Miyashita;Fuga Matsui;Hidenori Deto;Tatsuya Imai;N. Kondo
评价稀有物种的空间遗传结构是重要的，因为它可以提供栖息地破碎化程度和有效种群规模的信息，这是保护规划所必需的。我们利用MIG‐seq方法获得的单核苷酸多态性研究了近几十年来严重下降的草原蝴蝶Plebejus argyrognomon的遗传结构。研究区位于日本中部的伊纳盆地南部，在这一地区的农田边缘草原和河堤上都有凤尾草的生长。遗传距离与地理距离呈正相关，但在遗传距离大于12 km时变化不大。FST和结构分析表明，遗传分化明显。此外，遗传多样性与生境连通性指数呈正相关，表明孤立种群存在严重的遗传漂变。两种生境类型的遗传结构比较发现，农田边缘种群的遗传结构趋于相似，而河岸种群的遗传结构则不同，表明前者存在频繁的基因流动。再加上农田边缘存在许多栖息地斑块，我们认为研究地点北部的农田边缘种群对于确保该区域白杨种群的长期持久性是有价值的。
Evaluating the spatial genetic structure of rare species is important because it could provide information on the degree of habitat fragmentation and effective population size that are necessary for conservation planning. We examined the genetic structure of the grassland butterfly Plebejus argyrognomon that has severely declined in recent decades using single nucleotide polymorphisms obtained by the MIG‐seq method. The study region was in the southern Ina Basin located in central Japan, where P. argyrognomon inhabits grasslands in agricultural field margin as well as in riverbank. We found a positive association between genetic and geographic distances, but the pattern was nonlinear, with little change in genetic distance above 12 km. The genetic differentiation was considerable, as demonstrated by FST and Structure. Moreover, genetic diversity was positively associated with habitat connectivity index, suggesting severe genetic drift in isolated populations. Comparing two habitat types, the genetic structure tended to be similar among agricultural field margin populations while dissimilar among riverbank populations, suggesting frequent gene flow in the former. Coupled with the presence of many habitat patches in agricultural field margin, we consider that agricultural field margin populations in the northern part of the study sites appear valuable to ensure long‐term persistence of the regional P. argyrognomon population.