[Sanshensancao mixture alleviates the histologic injury and reduces the level of inflammatory factors of colonic mucosa in rats with ulcerative colitis].
[Sanshensancao mixture alleviates the histologic injury and reduces the level of inflammatory factors of colonic mucosa in rats with ulcerative colitis].
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发表时间：
2018-10
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通讯作者：
Jipan Liu;Wei Sun;Xue-zheng Zhao;Bingmao Li;Chengjun Wang
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作者：
Jipan Liu;Wei Sun;Xue-zheng Zhao;Bingmao Li;Chengjun Wang
目的探讨三参三草合剂对溃疡性结肠炎（UC）大鼠结肠黏膜组织学损伤及IL-12 P70、TNF-α、IFN-γ水平的影响。方法将大鼠随机分为正常对照组、模型组和三参三草合剂组，每组10只。对照组给予常规饲料喂养，三参三草合剂组和模型组采用三硝基苯磺酸钠（TNBS）-乙醇诱导法建立UC模型。然后给三参三草合剂组灌胃给予三参三草合剂4.5g/（kg.d），模型组灌胃给予等量生理盐水。治疗结束后进行结肠黏膜组织损伤（TDI）评分，ELISA法检测结肠黏膜IL-12 P70、TNF-α、IFN-γ水平。结果与模型组比较，三参三草合剂组TDI评分明显降低，IL-12 P70、TNF-α、IFN-γ水平明显降低。结论三参三草合剂可减轻UC大鼠结肠黏膜组织学损伤，降低IL-12 P70、TNF-α和IFN-γ水平。
Objective To investigate the effect of sanshensancao mixture on the histologic injury of colonic mucosa and the level of IL-12P70, TNF-α and IFN-γ in rats with ulcerative colitis(UC). Methods The rats were randomly divided into control group, model group and sanshensancao mixture group, with 10 rats in each group. The control group was fed with conventional diet, the sanshensancao mixture group and model group were established UC model by sodium trinitrobenzene sulfonate(TNBS)-ethanol induction method. Then 4.5 g /(kg.d) sanshensancao mixture was administered by gavage to the sanshensancao mixture group, and equal amount of normal saline was administered to the model group., After the treatment, all rats were evaluated for tissue damage injury (TDI) score of colon mucosa, and the levels of IL-12P70, TNF-α, and IFN-γ in the colon mucosa were measured by ELISA. Results Compared with the model group, the TDI score of the sanshensancao mixture group decreased significantly, and the level of IL-12P70, TNF-α and IFN-γ decreased significantly. Conclusion Sanshensancao mixture relieves the histological damage of colonic mucosa in rats with UC and reduces the level of IL-12P70, TNF-α and IFN-γ.