Facile oxygen exchanges of phosphoenolpyruvate and preparation of [18O]phosphoenolpyruvate.
Facile oxygen exchanges of phosphoenolpyruvate and preparation of [18O]phosphoenolpyruvate.
复制标题
磷酸烯醇丙酮酸的轻松氧交换和[18O]磷酸烯醇丙酮酸的制备。
DOI：
10.1021/bi00272a013
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Smith,LT
中科院分区：
文献类型：
--
作者：
O'NealJr,CC;Bild,GS;Smith,LT
克利福德角小奥尼尔，*·1加里S.磷酸烯醇式丙酮酸在酸性溶液中加热时，其磷酰基和羧基氧迅速交换，而烯醇式氧与水中的氧交换要慢得多。用气相色谱-质谱联用仪和磷-31核磁共振仪测定了98 ℃下在H2180中加热后lsO向磷酸烯醇式丙酮酸的转化。磷酸烯醇式丙酮酸的所有六个氧与水的交换速率随酸度的增加而增加，并且磷酰氧比羧基氧交换得更快。磷酰基的每个氧的交换速率比在1 NHC 1下的水解速率大16倍。这为[180]磷酸烯醇丙酮酸的合成提供了一种简便有效的方法，
Clifford C. O’Neal, Jr.,*· 1 Gary S. Bild, and Linda Tombras Smith abstract; Phosphoenolpyruvate when heated in acidic so-lution exchanges its phosphoryl and carboxyl oxygens rapidly and its enolic oxygen much more slowly with oxygens from water. The incorporationof lsO into phosphoenolpyruvate was measured by gas chromatography-mass spectrometry and phosphorus-31 nuclear magnetic resonance after heating in H2180 at 98 C. The rates of exchange of all six oxygens of phosphoenolpyruvate with water increase with increasing acidity, and the phosphoryl oxygens exchange more rapidly than the carboxyl oxygens. The rate of exchange of each oxygen of the phosphoryl group is 16-fold greater than the hydrolysis rate at 1 Nhc1. This provides a simple and useful method for the synthesis of [180] phosphoenolpyruvate highly