NATURAL POLYAMINES STIMULATE G-PROTEINS
NATURAL POLYAMINES STIMULATE G-PROTEINS
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DOI：
10.1042/bj2820545
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发表时间：
1992-03-01
影响因子：
4.1
通讯作者：
LANDRY, Y
中科院分区：
文献类型：
--
作者：
BUEB, JL;DASILVA, A;LANDRY, Y
天然多胺精胺和亚精胺、生物合成前体腐胺及其类似物尸胺和酪胺刺激来自小牛脑的重组成磷脂囊泡的纯化GTP结合蛋白（G（o）/G（i））的GTP酶活性。 其效价顺序为精胺>亚精胺>腐胺=尸胺>酪胺。 对这一观察结果的生理相关性进行了评估，结果显示多胺在刺激腹膜和气管大鼠肥大细胞中的效力顺序相同。 苯扎氯铵或百日咳毒素预处理2 h可抑制多胺对大鼠肥大细胞的激活。 多胺引起的磷酸肌醇的增加也被百日咳毒素抑制。 因此，我们认为细胞内多胺可能控制第二信使的基础水平，并通过G蛋白偶联受体调节细胞外信号转导。
The natural polyamines spermine and spermidine, the biosynthetic precursor putrescine and their analogues cadaverine and tyramine stimulate the GTPase activity of purified GTP-binding proteins (G(o)/G(i)) from calf brain reconstituted into phospholipid vesicles. The order of potency was spermine > spermidine > putrescine = cadaverine > tyramine. The physiological relevance of this observation was assessed, showing the same order of potency of polyamines in the stimulation of peritoneal and tracheal rat mast cells. The activation of rat mast cells by polyamines was inhibited by benzalkonium chloride or by a 2 h pretreatment of the cells with pertussis toxin. The increase in inositol phosphates evoked by polyamines was also inhibited by pertussis toxin. Therefore we propose that intracellular polyamines might control the basal level of second messengers and modulate extracellular signals transduced through G-protein-coupled receptors.