The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100
The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100
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DOI：
10.1016/s0147-619x(02)00120-8
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发表时间：
2002-09-01
期刊：
影响因子：
2.6
通讯作者：
Thierbach, G
中科院分区：
文献类型：
--
作者：
Tauch, A;Götker, S;Thierbach, G
我们确定了谷氨酸棒杆菌 LP-6 的 27.8 kb R 质粒 pTET3 的完整核苷酸序列，它编码链霉素、壮观霉素和四环素抗性。 pTET3的抗生素抗性决定簇包含intII样基因。它被插入序列 IS6100 和新型氨基糖苷腺苷转移酶基因盒 aadA9 截短。推导的 AADA9 蛋白与铜绿假单胞菌整合子 In51 的 AADA6 具有 61% 的同一性和 71% 的相似性。此外。 pTET3 携带新型阻遏物调节的四环素抗性决定簇 Tet 33，其揭示了与 I 组四环素外排系统的氨基酸序列同源性。观察到来自谷氨酸棒杆菌质粒 pAGI 的四环素流出蛋白 TetA(Z) 具有最高水平的相似性，其中氨基酸有 65% 相同和 77% 相似。 pTET3 的每个抗生素抗性区域的两侧都有最初在偶然分枝杆菌中发现的广泛插入序列 IS6100 的相同副本。对 IS6100 克隆副本进行的转座测定表明，该元件在谷氨酸棒杆菌中具有转座活性。这些数据表明，IS6100 通过介导携带抗性基因的 DNA 片段的共整合组装，在 pTET3 的进化史上发挥着核心作用。 (C) 2002 年爱思唯尔科学（美国）。版权所有。
We determined the complete nucleotide sequence of the 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum LP-6 which encodes streptomycin, spectinomycin, and tetracycline resistance. The antibiotic resistance determinant of pTET3 comprises an intIl-like gene. which was truncated by the insertion sequence IS6100, and the novel aminoglycoside adenyltransferase gene cassette aadA9. The deduced AADA9 protein showed 61%, identity and 71% similarity to AADA6 of integron In51 from Pseudomonas aeruginosa. In addition. pTET3 carries the novel repressor-regulated tetracycline resistance determinant Tet 33 which revealed amino acid sequence homology to group I tetracycline efflux systems. The highest level of similarity was observed to the tetracycline efflux protein TetA(Z) from the C glutamicum plasmid pAGI with 65% identical and 77%, similar amino acids. Each antibiotic resistance region of pTET3 is flanked by identical copies of the widespread insertion sequence IS6100 initially identified in Mycobacterium fortuitum. Transposition assays with a cloned copy of IS6100 revealed that this element is transpositionally active in C glutamicum. These data suggest a central role of IS6100 in the evolutionary history of pTET3 by mediating the cointegrative assembly of resistance gene-carrying DNA segments. (C) 2002 Elsevier Science (USA). All rights reserved.