Idiotype-like determinants on human T lymphocytes alloactivated in mixed lymphocyte culture
Idiotype-like determinants on human T lymphocytes alloactivated in mixed lymphocyte culture
复制标题
混合淋巴细胞培养物中同种激活的人 T 淋巴细胞的独特型样决定簇
DOI：
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发表时间：
1982
影响因子：
15.3
通讯作者：
D. W. King
中科院分区：
文献类型：
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作者：
N. Suciu;C. Rohowsky;P. Kung;D. W. King
人类白细胞抗原DR不同刺激物在5-d MLC中激活的T细胞具有刺激自体混合淋巴细胞反应(AMLR)的能力。我们已经证明，同种异体致敏T细胞对AMLR的激活是由刺激同种异体抗原的独特型受体的表达决定的。这一点已经在实验中得到证明，在这些实验中，纯化的OKT-3阳性T细胞悬液首先与AMLR激活的T淋巴母细胞一起预刺激9d，然后在二次AMLR中与对各种HLA-DR同种异体抗原致敏的自体淋巴母细胞进行试验。只有对与主要AMLR刺激物相同的HLA-DR特异性致敏的自体淋巴母细胞才能诱导加速记忆反应。这种反应不被识别Ia类决定簇的鼠单抗抑制，也不被人类同种异体静息的外周血淋巴细胞触发。因此，同种异体激活的T淋巴母细胞在继发性AMLR中的识别似乎是由自体刺激物表达的独特型样决定因素所特有的。
T cells alloactivated in 5-d MLC with an HLA-DR-different stimulator acquire the capacity of stimulating the autologous mixed lymphocyte response (AMLR). We have demonstrated that activation of AMLR by allosensitized T cells is determined by the expression of the idiotype receptor for the stimulating HLA-DR alloantigen. This has been shown in experiments in which purified, OKT-3-positive T cell suspensions were first primed for 9 d with AMLR-activated T lymphoblasts, then tested in secondary AMLR with autologous lymphoblasts sensitized to various HLA- DR alloantigens. Accelerated memory responses were induced only by autologous lymphoblasts that had been sensitized against the same HLA- DR specificity as the primary AMLR stimulators. This response was not inhibited by a mouse monoclonal antibody recognizing Ia-like determinants, and was not triggered by human allogeneic resting peripheral blood lymphocytes. Thus, recognition of alloactivated T lymphoblasts in secondary AMLR seems to be specific for the idiotype- like determinants expressed by the autologous stimulators.