Detergent resistance as a tool in membrane research
Detergent resistance as a tool in membrane research
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DOI：
10.1038/nprot.2007.294
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Simons, Kai
中科院分区：
文献类型：
--
作者：
Lingwood, Daniel;Simons, Kai
生物膜是一种复杂的基质，其中存在不同的脂质环境。更有序或筏环境已被认为是通过其相对耐洗涤剂的生化可访问。本文概述了在我们的实验室开发的协议，用于分析这种耐洗涤剂的膜（DRM）。我们强调这样一个事实，即DRM是人为的性质，不应该模棱两可的脂筏，其用途仅限于分配筏协会的潜力最令人信服的DRM组合物的变化时，引起的生化/生理相关的事件。这些协议在1-2天内完成。
The biological membrane is a complicated matrix wherein different lipid environments are thought to exist. The more ordered or raft environment has been perceived biochemically accessible via its relative resistance to detergent. This paper outlines the protocols developed in our laboratory for the analysis of such detergent-resistant membranes (DRMs). We stress the fact that DRMs are artifactual in nature and should not be equivocated to lipid rafts, their usefulness being limited to assigning raft-association potential most convincingly when changes in DRM composition are induced by biochemically/physiologically relevant events. These protocols are completed in 1-2 d.