The isolation and characterization of myostatin gene in Japanese flounder (Paralichthys olivaceus): Ubiquitous tissue expression and developmental specific regulation
The isolation and characterization of myostatin gene in Japanese flounder (Paralichthys olivaceus): Ubiquitous tissue expression and developmental specific regulation
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DOI：
10.1016/j.aquaculture.2008.04.015
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发表时间：
2008-08
期刊：
影响因子：
4.5
通讯作者：
Qiwang Zhong;Quanqi Zhang;Yanjie Chen;Yeying Sun;J. Qi;Zhigang Wang;S. Li;Chunmei Li;Xun Lan-Xun
中科院分区：
文献类型：
--
作者：
Qiwang Zhong;Quanqi Zhang;Yanjie Chen;Yeying Sun;J. Qi;Zhigang Wang;S. Li;Chunmei Li;Xun Lan-Xun
肌肉生长抑制素 (MSTN) 是骨骼肌生长和发育的负调节因子。在当前的研究中，MSTN 基因是从一种比目鱼——日本牙鲆 (Paralichthys olivaceus) 中克隆出来的。在 5520 bp 基因组序列中，鉴定出三个外显子、两个内含子以及 5' 和 3' 侧翼序列。推定的氨基酸序列长 377 个残基，包括一个信号肽、九个保守的半胱氨酸残基和一个 RXXR 蛋白水解加工位点。 P. olivaceus MSTN (pMSTN) 的整体氨基酸序列与其他生物体的氨基酸序列高度保守。系统发育分析表明 pMSTN 与其他已知的 MSTN 和 GDF-11 基因的进化关系。在非编码区鉴定出 4 个微卫星序列，在编码区鉴定出 19 个 SNP。表达分析显示，pMSTN基因不仅在骨骼肌中表达，还在软骨等其他组织中表达，此前在鱼类中未见报道。尽管 pMSTN 在鳃中表达，但在 FG-9307（来自 P. olivaceus 的连续鳃细胞系）中未检测到转录本。定量RT-PCR分析表明pMSTN的表达似乎受到发育调节。在未受精卵中检测到的 pMSTN mRNA 表明母体沉积。考虑到pMSTN在牙鲆中的广泛组织分布和整个胚胎发育过程中的持续表达，鱼类中的MSTN基因与哺乳动物中的MSTN基因可能发挥不同的作用。
Myostatin (MSTN) is a negative regulator of skeletal muscle growth and development. In the current study, the MSTN gene was cloned from one of flatfish species, Japanese flounder (Paralichthys olivaceus). In the 5520-bp genomic sequence, three exons, two introns, and 5' and 3' flanking sequences were identified. The putative amino acid sequence was 377 residues long, including a signal peptide, nine conserved cysteine residues, and a RXXR proteolytic processing site. The overall amino acid sequence of the P. olivaceus MSTN (pMSTN) was highly conserved with that of other organisms. Phylogenetic analysis suggested the evolutionary relationships of pMSTN with other known MSTN and GDF-11 genes. There were four microsatellite sequences identified in noncoding regions and 19 SNPs identified in coding sequences. Expression analysis revealed that pMSTN gene was expressed not only in the skeletal muscle, but also in other tissues, including cartilage, which has not been reported in fish previously. Although pMSTN was expressed in gill, no transcript could be detected in FG-9307, a continuous gill cell line from P. olivaceus. Quantitative RT-PCR analysis indicated that the expression of pMSTN appeared to be developmentally regulated. The pMSTN mRNA detected in unfertilized eggs indicated the maternal deposit. Considering of the ubiquitous tissue distribution and the continuous expression of pMSTN during the whole embryonic development in Japanese flounder, MSTN gene in fish may play different roles in comparison with that in mammals.