NF-IL6 represses early gene expression of human papillomavirus type 16 through binding to the noncoding region
NF-IL6 represses early gene expression of human papillomavirus type 16 through binding to the noncoding region
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NF-IL6 通过与非编码区结合抑制人乳头瘤病毒 16 型的早期基因表达
作者：
Satoru Kyo;Masahiro Inoue;Yukihiro Nishio;Kazuyoshi Nakanishi;Shizuo Akira;H. Inoue;M. Yutsudo;Osamu Tanizawa;Akira Hakura
人乳头瘤病毒16型（HPV 16）早期基因（包括E6和E7转化基因）的表达受与非编码区（NCR）结合的几种细胞因子（如糖皮质激素受体、NF-1和AP 1）的调控，这些因子均为正调控因子。我们证明了白细胞介素6表达核因子（NF-IL 6）特异性结合HPV 16 NCR范围从核苷酸7007至7766，并抑制HPV 16的早期基因表达。HPV 16 NCR中的反应元件在核苷酸7454至7766的区域内确定。在该区域，已检测到许多其他细胞反式激活因子（如NF-1和AP 1）的结合位点。有趣的是，在该区域中，NF-1的7个结合位点中的3个和AP 1的2个结合位点中的2个与通过计算机分析鉴定的推定的NF-IL 6结合位点重叠。用含有这种NF-Ⅰ或AP-1位点的寡核苷酸进行的竞争实验表明，NF-IL-6确实与它们结合。此外，在使用突变型NF-1 L 6表达载体的氯霉素乙酰转移酶测定中，NF-1 L 6的DNA结合结构域被证明是阻遏所必需的，而功能结构域不是。这些发现表明抑制可能是由与其他转录激活因子如NF-1和AP 1的竞争引起的。因此，NF-IL 6可能在作为宿主对病毒感染的抗性的一部分的病毒转录的调节中起重要作用。
The expression of human papillomavirus type 16 (HPV16) early genes, including E6 and E7 transforming genes, is regulated by several cellular factors binding to the noncoding region (NCR), such as the glucocorticoid receptor, NF-I, and AP1, all of which are positive regulators. We demonstrated that the nuclear factor for interleukin 6 expression (NF-IL6) specifically binds to the HPV16 NCR ranging from nucleotides 7007 to 7766 and represses the early gene expression of HPV16. The responsive element in HPV16 NCR was determined within the region ranging from nucleotides 7454 to 7766. In this region, many binding sites for other cellular transactivators, such as NF-I and AP1, have been detected. Interestingly, three of seven binding sites for NF-I and two of two binding sites for AP1 in this region overlap with the putative NF-IL6 binding sites identified by computer analysis. Competition experiments with the oligonucleotides containing such NF-I or AP1 sites indicated that NF-IL6 certainly binds to them. Furthermore, in a chloramphenicol acetyltransferase assay using mutant NF-IL6 expression vectors, the DNA binding domain of NF-IL6 was shown to be necessary for repression, whereas the functional domain was not. These findings indicate that repression may be caused by competition with other transcriptional activators, such as NF-I and AP1. Thus, NF-IL6 may play a significant role in the regulation of viral transcription as a part of the host's resistance to viral infection.
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癌基因jun和核信号传导。
影响因子：
13.8
作者：
Vogt,PK;Bos,TJ
通讯作者：
Bos,TJ
影响因子：
--
作者：
Peter K. Vogt;Bos Tj
通讯作者：
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影响因子：
10.5
作者：
S. C. Williams;Carrie A. Cantwell;P. Johnson
通讯作者：
S. C. Williams;Carrie A. Cantwell;P. Johnson
影响因子：
56.9
作者：
DYSON, N;HOWLEY, PM;HARLOW, E
通讯作者：
HARLOW, E
通过含有不同细胞依赖性和 AP-1 响应模块的 88 核苷酸增强子对人乳头瘤病毒 16 P97 启动子进行转录激活。
DOI：
--
发表时间：
1990
期刊：
The New biologist
影响因子：
--
作者：
Cripe,TP;Alderborn,A;Anderson,RD;Parkkinen,S;Bergman,P;Haugen,TH;Pettersson,U;Turek,LP
通讯作者：
Turek,LP