Real-Time PCR Targeting the penA Mosaic XXXIV Type for Prediction of Extended-Spectrum-Cephalosporin Susceptibility in Clinical Neisseria gonorrhoeae Isolates.
Real-Time PCR Targeting the penA Mosaic XXXIV Type for Prediction of Extended-Spectrum-Cephalosporin Susceptibility in Clinical Neisseria gonorrhoeae Isolates.
复制标题
针对 penA Mosaic XXXIV 型的实时 PCR 预测临床淋病奈瑟菌分离株中的超广谱头孢菌素敏感性。
DOI：
10.1128/aac.01339-17
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发表时间：
2017
影响因子：
4.9
通讯作者：
Klausner,JD
中科院分区：
文献类型：
--
作者：
Wong,LK;Hemarajata,P;Soge,OO;Humphries,RM;Klausner,JD
对超广谱头孢菌素（ESC）敏感性降低的淋球菌分离株正在增加。我们开发了一种方法来预测N。通过靶向penAmosaic XXXIV（一种在ESC MIC升高的美国分离株中普遍存在的等位基因）检测淋病对ESC的敏感性。该检测方法在预测临床分离株中至少一种ESC MIC高于CDC警戒值时的敏感性为97%，特异性为100%，并且可以与先前验证的gyrAPCR多重结合来预测环丙沙星的敏感性。
Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum cephalosporins (ESCs) are increasing. We developed an assay to predict N. gonorrhoeae susceptibility to ESCs by targetingpenAmosaic XXXIV, an allele prevalent among U.S. isolates with elevated ESC MICs. The assay was 97% sensitive and 100% specific for predicting at least one ESC MIC above the CDC alert value among clinical isolates, and it could be multiplexed with a previously validatedgyrAPCR to predict ciprofloxacin susceptibility.