Noninvasive tests for diagnosis of Chlamydia trachomatis infection: application of ligase chain reaction to first-catch urine specimens of women.
Noninvasive tests for diagnosis of Chlamydia trachomatis infection: application of ligase chain reaction to first-catch urine specimens of women.
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诊断沙眼衣原体感染的无创检测：连接酶链反应在女性首次尿液标本中的应用。
DOI：
10.1093/infdis/172.5.1411
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Lee,HH
中科院分区：
文献类型：
--
作者：
Schachter,J;Moncada,J;Whidden,R;Shaw,H;Bolan,G;Burczak,JD;Lee,HH
应用连接酶链反应（LCR）对4053例女性首采尿（FCU）标本进行了沙眼衣原体感染的诊断研究。结果与宫颈（所有）和尿道（2812例妇女）细胞培养（TC）分离的结果进行了比较。参考标准为直接荧光抗体试验证实的衣原体质粒DNA阳性或另一种衣原体基因的LCR。与宫颈培养比较，LCR的敏感性为88.2%，特异性为100%。增加尿道培养使TC敏感性从67.1%增加到74%，使LCR敏感性降低到85.9%。FCU标本双培养法衣原体感染率为5%（142/2812），LCR法为5.9%（165/2812）。FCU标本LCR对诊断衣原体感染具有高度敏感性和特异性。它比TC更敏感，很可能为公共卫生当局提供一种有用的无创筛查测试，用于无症状女性的衣原体感染。
Ligase chain reaction (LCR) to diagnoseChlamydia trachomatisinfection was evaluated using first-catch urine (FCU) specimens from 4053 women. Results were compared with those of cell culture (TC) isolation from cervix (all) and urethra (2812 women). The reference standard was TC positivity or positive LCR for chlamydial plasmid DNA confirmed by direct fluorescent antibody test or LCR for another chlamydial gene. Compared with cervical culture, LCR was 88.2% sensitive and 100% specific. Adding urethral culture increased TC sensitivity from 67.1% to 74% and reduced LCR sensitivity to 85.9%. The prevalence of chlamydial infection was 5% (142/2812) by the dual culture system and 5.9% (165/2812) by LCR on FCU specimens. LCR on FCU specimens is highly sensitive and specific for diagnosing chlamydial infection. It is more sensitive than TC and may well present public health authorities with a useful noninvasive screening test for chlamydial infection in asymptomatic women.