Isolation and characterisation of Aspergillus awamori BS05, a root-knot-nematode-trapping fungus
Isolation and characterisation of Aspergillus awamori BS05, a root-knot-nematode-trapping fungus
复制标题
泡盛曲霉 BS05（一种根结线虫捕获真菌）的分离和表征
DOI：
10.1080/09583157.2015.1040373
复制
发表时间：
2015-01-01
影响因子：
1.4
通讯作者：
Sun, Xiaotang
中科院分区：
文献类型：
--
作者：
Cui, Ruqiang;Fan, Chengming;Sun, Xiaotang
捕食线虫真菌是一种重要的生物防治剂，可通过粘附或机械菌丝诱捕器来防治寄生线虫。根据形态特征和内转录间隔区DNA序列的分子特征，对一株番茄根际土壤中的捕食根结线虫真菌泡盛曲霉进行了鉴定。分生孢子头白色至黑褐色，松散球形，直径72-127 μm。分生孢子梗通常产生于基生菌丝的足细胞，直，960-1730 × 10.2-13.4 μm，透明至淡褐色，囊泡以下不收缩;囊泡半球形至细长，直径43-56 μm，黑褐色，上半部至三分之二可育。鳃为双列，后叶为12-26 × 3.8-4.7 μm，瓶梗为8.2-9.4 × 2.5-3 μm。分生孢子球形或近球形，直径3.6-4.8 μm，粗糙，灰褐色，平行成链。A.泡盛BS 05对南方根结线虫的盆栽防效为44.9%，是一种有潜力的生防药剂。这是关于A的第一篇报道。泡盛作为捕食线虫的真菌。
Nematode-trapping fungi are important biocontrol agents against parasitic nematodes through adhesive or mechanical hyphal traps. Aspergillus awamori, a root-knot-nematode-trapping fungus from tomato rhizosphere soil, was identified based on morphology and molecular characteristics of internal transcribed spacer DNA sequence. Conidial heads were white to black brown, loosely globose, and 72–127 μm in diameter. Conidiophores usually arose from the foot cell of basal mycelium, straight, and 960–1730 × 10.2–13.4 μm, hyaline to pale brown, not constricted below the vesicles; vesicles hemispherical to elongate, 43–56 μm in diameter, black brown, fertile over the upper half to two-thirds. Aspergilla were biseriate, and metulae were variable, 12–26 × 3.8–4.7 μm; phialides were 8.2–9.4 × 2.5–3 μm. Conidia were globose or subglobose, 3.6–4.8 μm in diameter, rough, grey brown and parallel in chains. A. awamori BS05 showed 44.9% control efficacy against Meloidogyne incogtina in pot experiments which suggests it as a potential biocontrol agent against Meloidogyne. This is the first report on A. awamori as nematode-trapping fungus.