Integration of mild acid hydrolysis in γ-valerolactone/water system for enhancement of enzymatic saccharification from cotton stalk.
Integration of mild acid hydrolysis in γ-valerolactone/water system for enhancement of enzymatic saccharification from cotton stalk.
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DOI：
10.1016/j.biortech.2015.09.111
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发表时间：
2016
影响因子：
11.4
通讯作者：
Miao Wu;Zhong Yan;X. Zhang;Feng Xu;R. Sun
中科院分区：
文献类型：
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作者：
Miao Wu;Zhong Yan;X. Zhang;Feng Xu;R. Sun
采用γ-戊内酯（GVL）/水体系进行弱酸水解，并结合酶解提高酶解糖化效率。进行了可溶性糖的定量分析和固体残留物的结构表征。结果表明，水相中的可溶性糖主要由木糖和葡萄糖的单体和低聚物组成，其含量取决于GVL与水的比例。此外，由于预处理强度适中，几乎未检测到抑制剂。与未经处理的原料相比，预处理样品的酶解产率增加了2倍，与80/20 GVL/H2O的混合物。结合溶解在水相中的葡萄糖量（14.6%），棉秆中92.6%以上的葡萄糖被释放和回收。综合分析，GVL/H2O体系处理为生物质制糖提供了一条更为有效的途径。
In this study, mild acid hydrolysis using γ-valerolactone (GVL)/water system integrated with enzymatic hydrolysis was carried out for the enhancement of enzymatic saccharification efficiency. The quantitative analysis of soluble carbohydrates and structural characterizations of solid residues were conducted. Results showed that the soluble carbohydrates in the water-phase were mainly composed of monomers and oligomers from xylose and glucose, while the contents of which were depended on the ratio of GVL to water. Moreover, the inhibitors were hardly detected due to the moderate pretreatment severity. Compared with the untreated feedstock, the yields of enzymatic hydrolysis from pretreated samples increased by two-fold with the mixture of 80/20 GVL/H2O. Combined with the amount of glucose (14.6%) dissolved in the water-phase, over 92.6% of glucose in cotton stalk was released and recovered. Based on the comprehensive analysis, treatment with GVL/H2O system provided us a more effective approach for sugar production from biomass.