Development of an immunochromatographic strip test for the rapid simultaneous detection of deoxynivalenol and zearalenone in wheat and maize
Development of an immunochromatographic strip test for the rapid simultaneous detection of deoxynivalenol and zearalenone in wheat and maize
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开发用于快速同时检测小麦和玉米中脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的免疫层析试纸条测试
DOI：
10.1016/j.foodcont.2012.04.035
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发表时间：
2012-11-01
期刊：
影响因子：
6
通讯作者：
He, Qing-Hua
中科院分区：
文献类型：
--
作者：
Huang, Zhi-Bing;Xu, Yang;He, Qing-Hua
开发了一种基于竞争格式的胶体金免疫层析试纸条 (ICS) 测试，用于快速同时检测小麦和玉米样品中的脱氧雪腐镰刀菌烯醇 (DON) 和玉米赤霉烯酮 (ZEN)。对于金基ICS测试，将抗原（DON-CBSA和ZEN-BSA）和羊抗小鼠IgG分别绘制在NC膜上作为两条测试线（T-1和T-2线）和对照线（C线）。将单克隆抗体 (MAb)-金缀合物（抗 DON MAb-金和抗 ZEN MAb-金）喷涂到缀合物垫上。为了进行测试，将 5 g 样品与 50% 甲醇水 (v/v) 以 1:5 的比例提取，摇动 3 分钟，提取物直接使用，无需进一步净化步骤。用缓冲液将水提取物稀释 2.5 倍，消除基质干扰。 ICS测试的DON和ZEN的截止水平分别为100ng/mL（1000μg/kg）和6ng/mL（60μg/kg），并且可以在5分钟内完成。对小麦和玉米样品中 DON 和 ZEN 的分析表明，ICS 测试获得的数据与 ELISA 和仪器分析（GC/MS 用于 DON 测定，HPLC 用于 ZEN 测定）获得的数据非常一致。结果表明，ICS 测试可以作为一种可靠、快速、经济且方便的现场筛选技术定性工具，无需特殊仪器即可同时测定小麦和玉米样品中的 DON 和 ZEN。 (C) 2012 Elsevier Ltd. 保留所有权利。
A colloidal gold immunochromatographic strip (ICS) test based on competitive format was developed for the rapid simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN) in wheat and maize samples. For gold-based ICS test, antigen (DON-CBSA and ZEN-BSA) and goat anti-mouse IgG were respectively drawn on NC membrane as two test line (T-1 and T-2 line) and control line (C line), respectively. Monoclonal antibody (MAb)-gold conjugates (anti-DON MAb-gold and anti-ZEN MAb-gold) were sprayed onto the conjugate pad. To perform the test, 5 g of sample was extracted in a ratio of 1:5 with 50% methanol water (v/v) by shaking for 3 min and the extract directly used without further cleanup steps. Matrix interference was eliminated by 2.5-fold dilutions of water extracts with buffer. The ICS test, which has cut-off levels of 100 ng/mL (1000 mu g/kg) and 6 ng/mL (60 mu g/kg) for DON and ZEN, respectively, and can be completed in 5 min. Analysis of DON and ZEN in wheat and maize samples revealed that data obtained from ICS test were in a good agreement with those obtained from ELISA and instrumental analysis (GC/MS for DON determination and HPLC for ZEN determination). The results demonstrate that the ICS test can be used as a reliable, rapid, cost-effective and convenient qualitative tool for on-site screening technique for the simultaneous determination of DON and ZEN in wheat and maize samples without special instrumentation. (C) 2012 Elsevier Ltd. All rights reserved.