Interleukin 1β, interleukin 6, β2-microglobulin, and transforming growth factor-α in gingival crevicular fluid from human periodontal disease
Interleukin 1β, interleukin 6, β2-microglobulin, and transforming growth factor-α in gingival crevicular fluid from human periodontal disease
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DOI：
10.1016/s0003-9969(99)00020-5
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发表时间：
1999-06-01
影响因子：
3
通讯作者：
Kojima, K
中科院分区：
文献类型：
--
作者：
Mogi, M;Otogoto, J;Kojima, K
炎症介质是牙周病发病机制的核心，可作为诊断的标志物。本研究的目的是确定和定量的各种生长因子，牙周炎相关的修饰剂[可溶性形式的Fas（sFas）和bcl-2]和细胞因子在龈沟液（GCF）的患者与不同严重程度的牙周炎与对照组相比。GCF样本取自牙周病患者和对照组。采用酶联免疫吸附法检测表皮生长因子、转化生长因子（TGF）-α、白细胞介素（IL）-1 β、IL-6、干扰素-γ、β 2-微球蛋白（β 2-MG）、凋亡相关因子sFas和bcl-2的含量。牙周病患者的TGF-α显著低于对照组。相比之下，IL-1 β、IL-6和β 2-MG的浓度在重度牙周病组中显著高于对照组。GCF中的总蛋白量在疾病组显著高于对照组（p < 0.05）。TGF-α、IL-1 β和β 2-MG浓度与疾病严重程度（囊袋深度）和炎症（探测时出血）的临床指标相关（斯皮尔曼等级相关，r < 0.05）。凋亡相关的修饰物（sFas和bcl-2）不能在任何样品中检测到。这些结果表明，生长因子TGF-α和某些细胞因子与牙周病的存在有关。(C)1999由Elsevier Science Ltd.出版。保留所有权利。
Inflammatory mediators are central to the pathogenesis of periodontal diseases and may be used as markers in diagnosis. The aim of this study was to identify and quantify the various growth factors, apoptosis-related modifiers [soluble form of Fas (sFas) and bcl-2] and cytokines in the gingival crevicular fluid (GCF) of patients with different severities of periodontitis as compared with those of controls. GCF samples were taken from patients with periodontal disease and from controls. The concentrations of epidermal growth factor, transforming growth factor (TGF)-alpha, interleukin (IL)-1 beta, IL-6, interferon-gamma, beta(2)-microglobulin (beta(2)-MG), and apoptosis-related modifiers sFas and bcl-2 in the samples were determined by enzyme-linked immunosorbent assay. TGF-alpha was significantly lower in patients with periodontal disease than in the controls. In contrast, the concentrations of IL-1 beta, IL-6, and beta(2)-MG were significantly higher in the group with severe periodontal disease than in the controls. The amount of total protein in the GCF was considerably higher in the disease group than the controls (p < 0.05). TGF-alpha, IL-1 beta, and beta(2)-MG concentrations were associated (Spearman rank correlation, r < 0.05 for all) with clinical measures of disease severity (pocket depth) and inflammation (bleeding when probed). Apoptosis-related modifiers (sFas and bcl-2) could not be detected in any samples. These results suggest that the growth factor TGF-alpha and certain cytokines are associated with the presence of periodontal disease. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.