Identification and analysis of plasticity-induced late-response genes
Identification and analysis of plasticity-induced late-response genes
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DOI：
10.1073/pnas.0305170101
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发表时间：
2004-02-17
影响因子：
11.1
通讯作者：
Dawson, TM
中科院分区：
文献类型：
--
作者：
Hong, SJ;Li, HW;Dawson, TM
兴奋性神经递质谷氨酸激活N-甲基-D-天冬氨酸（NMDA）受体，通过基因表达的改变诱导持久的突触变化。据信，这些持久的变化有助于学习和记忆、药物耐受和缺血预处理。为了识别NMDA诱导的迟发反应基因，我们使用了强大的基因识别方法、初级cDNA库表达差异分析（DAzLE）和来自初级皮质神经元的cDNA微阵列。我们在这里报告的各种基因，我们命名为可塑性诱导基因（PLINGs），上调与NMDA受体激活后的差异表达模式，表明有一个广泛的和动态范围的持久的神经元反应，通过NMDA受体激活发生。我们的研究结果提供了一个分子解剖的活动依赖性的持久的神经元反应诱导的NMDA受体激活。
The excitatory neurotransmitter, glutamate, activates N-methyl-D-aspartate (NMDA) receptors to induce long-lasting synaptic changes through alterations in gene expression. It is believed that these long-lasting changes contribute to learning and memory, drug tolerance, and ischemic preconditioning. To identify NMDA-induced late-response genes, we used a powerful gene-identification method, differential analysis of primary cDNA library expression (DAzLE), and cDNA microarray from primary cortical neurons. We report here that a variety of genes, which we have named plasticity-induced genes (PLINGs), are up-regulated with differential expression patterns after NMDA receptor activation, indicating that there is a broad and dynamic range of long-lasting neuronal responses that occur through NMDA receptor activation. Our results provide a molecular dissection of the activity-dependent long-lasting neuronal responses induced by NMDA receptor activation.