The humoral immune response of turbot to recently isolated pathogenic Enterococcus strains. Cross-reactivity with other Gram-positive bacteria
The humoral immune response of turbot to recently isolated pathogenic Enterococcus strains. Cross-reactivity with other Gram-positive bacteria
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DOI：
10.1016/0378-1135(95)00139-5
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发表时间：
1996-01-01
影响因子：
3.3
通讯作者：
Ubeira, FM
中科院分区：
文献类型：
--
作者：
Leiro, J;Toranzo, AE;Ubeira, FM
最近在西班牙西北部发现一种在养殖大比目鱼（Scophthalmus maximus）中引起严重死亡的肠球菌。在酶联免疫吸附试验（ELISA）中发现，经腹腔免疫产生的针对新病原体RA-99.1分离物的特异性大菱鲆血清抗体与其他肠球菌或非肠球菌革兰氏阳性菌无交叉反应。在免疫印迹法中，针对菌株RA-99.1的抗体识别同源总可溶性抗原制剂（TSA）中的相同成分，与从不同农场获得的其他两株病原菌的TSA中的相同成分。抗ra -99.1血清也能识别其他几种革兰氏阳性细菌的TSA成分。免疫金标记表明，引起对该病原体体液免疫反应的抗原主要位于细菌表面。
An Enterococcus sp. causing severe mortalities among farmed turbot (Scophthalmus maximus) has recently been detected in northwest Spain. We found that specific turbot serum antibodies raised against isolate RA-99.1 of the new pathogen by intraperitoneal immunization, did not cross react in enzyme-linked immunosorbent assay (ELISA) with other enterococcal or non-enterococcal Grampositive bacteria. In immunoblotting, antibodies raised against strain RA-99.1 recognized the same components in the homologous total soluble antigen preparation (TSA) as in TSA of two other isolates of the pathogen obtained from different farms. Anti-RA-99.1 serum also recognized some components of the TSA of several other Gram-positive bacteria. Immunogold labelling indicated that the antigens which provoke the humoral immune response to this pathogen are located mainly on the bacterial surface.