ORIGIN OF RETINAL ASTROCYTES IN THE RAT - EVIDENCE OF MIGRATION FROM THE OPTIC-NERVE
ORIGIN OF RETINAL ASTROCYTES IN THE RAT - EVIDENCE OF MIGRATION FROM THE OPTIC-NERVE
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DOI：
10.1002/cne.902860305
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发表时间：
1989-08-15
影响因子：
2.5
通讯作者：
STONE, J
中科院分区：
文献类型：
--
作者：
LING, TL;MITROFANIS, J;STONE, J
为了验证最近关于视网膜星形胶质细胞起源的观点，我们研究了星形胶质细胞在发育中的白化病大鼠视网膜中的扩散。星形胶质细胞用其中间纤维胶质细胞酸性蛋白（GFAP）的抗体进行鉴定。星形胶质细胞首次检测到E（胚胎天）18，形成冠状突起周围的视盘。在随后的几天里，星形胶质细胞在视网膜上延伸，在出生时（通常为E21-22）覆盖约35%的视网膜，并在P（出生后第8天）到达视网膜的边缘。当它们扩散时，星形胶质细胞与发育中的血管系统密切相关，在未闭血管的前面以小而明显的边缘扩散。大多数周边星形胶质细胞呈双极形态，并向视网膜边缘延伸。靠近视盘的星形胶质细胞呈成熟细胞的星状。在E18时，视神经中出现1型星形胶质细胞后2天，视盘中出现星形胶质细胞（米勒等人：Dev. 111：35-41，“85），表明视网膜星形胶质细胞可能是在视神经中产生的1型星形胶质细胞。Watanabe和Raff（Nature 332：834-837，'' 88）最近报道了一项支持相同结论的独立研究。
To test recent ideas on the origin of retinal astrocytes, we have studied the spread of astrocytes in the developing retinal of the albino rat. Astrocytes were identified with antibodies to their intermediate filaments, glial fibrillary acidic protein (GFAP). Astrocytes were first detected at E(embryonic day)18, forming a corona of processes around the optic disc. Over subsequent days, astrocytes extended over the retina, covering approximately 35% of the retina at birth (typically E21-22) and reaching the edge of the retina by P(postnatal day)8. As they spread, astrocytes were closely associated with the developing vasculature, spreading ahead of patent vessels by a small but distinct margin. The most peripheral astrocytes assumed a bipolar morphology and extended processes towards the margin of the retina. Astrocytes nearer the optic disc showed the stellate shape characteristic of mature cells. The appearance of astrocytes at the optic disc at E18, 2 days after the appearance of type-1 astrocytes in the optic nerve (Miller et al.: Dev. Biol. 111:35-41, ''85), suggests that retinal astrocytes may be type-1 astrocytes generated in the optic nerve. Watanabe and Raff (Nature 332:834-837, ''88) have recently reported an independent study supporting the same conclusions.