Expression of RUNX3 protein in human esophageal mucosa and squamous cell carcinoma
Expression of RUNX3 protein in human esophageal mucosa and squamous cell carcinoma
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DOI：
10.1159/000091329
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发表时间：
2005-01-01
期刊：
影响因子：
5
通讯作者：
Ito, H
中科院分区：
文献类型：
--
作者：
Hiramatsu, T;Osaki, M;Ito, H
Runt-related transcriptional factor gene 3（RUNX 3）属于转录因子的runt结构域家族，其在正常组织发育和多种器官的肿瘤发生中起重要作用。本研究检测了RUNX 3蛋白在人食管粘膜和鳞状细胞癌中的表达，并与临床病理资料进行比较。Western blot和RT-PCR检测结果显示，RUNX 3/P44和P27在3株人食管鳞癌细胞系、3对食管鳞癌细胞系及相应的非肿瘤粘膜标本中均有表达，而P46无表达。RUNX 3在正常食管粘膜的棘细胞和功能细胞层细胞中表达。另一方面，免疫反应仅见于癌珠周围的癌细胞。RUNX 3在19例高分化SCC中的表达显著高于56例中分化SCC或69例低分化SCC（p < 0.01）。RUNX 3表达较低的29例患者的3年生存率显著低于表达较高的37例患者（p = 0.0003）。提示RUNX 3蛋白可能在食管黏膜和SCC的细胞分化中起重要作用。该表达与患者的生存期延长相关，这意味着在食管SCC中具有肿瘤抑制作用。版权所有（c）2005 S. Karger AG，巴塞尔。
Runt-related transcriptional factor gene 3 (RUNX3) belongs to the runt domain family of transcriptional factors that plays an important role during normal tissue development and in tumorigenesis in several organs. This study examined the expression of RUNX3 protein in human esophageal mucosa and squamous cell carcinoma in comparison with clinicopathological profiles. Western blot analysis and RT-PCR revealed that both RUNX3/P44 and P27, but not P46, were expressed in all three human esophageal squamous cell carcinoma (SCC) cell lines, as well as in three pairs of esophageal SCC cell lines and the corresponding nontumoral mucosa specimens. RUNX3 expression was shown in prickle and functional cell layer cells in normal esophageal mucosa. On the other hand, immunoreactivity was seen only in carcinoma cells around the cancer pearls. RUNX3 expression was significantly higher in the 19 well-differentiated SCCs than in the 56 moderately or 69 poorly differentiated SCCs (p < 0.01). The 3-year survival rate was significantly lower in the 29 patients with lower RUNX3 expression than in the 37 patients with higher expression (p = 0.0003). These results indicated that RUNX3 protein might play an important role in cellular differentiation in both esophageal mucosa and SCC. The expression correlated with the patients' prolonged survival, implying a tumor suppressive effect in esophageal SCCs. Copyright (c) 2005 S. Karger AG, Basel.