Pericellular Matrix of Decidua-Derived Mesenchymal Cells: A Potent Human-Derived Substrate for the Maintenance Culture of Human ES Cells
Pericellular Matrix of Decidua-Derived Mesenchymal Cells: A Potent Human-Derived Substrate for the Maintenance Culture of Human ES Cells
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DOI：
10.1002/dvdy.21944
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发表时间：
2009-05-01
影响因子：
2.5
通讯作者：
Sasai, Yoshiki
中科院分区：
文献类型：
--
作者：
Nagase, Tomoko;Ueno, Morio;Sasai, Yoshiki
在常规培养中，将人胚胎干（hES）细胞维持在饲养细胞或特殊培养基质如Matrigel上。然而，为了扩增用于临床应用的hES细胞，出于安全原因，期望在培养物中最小化动物来源的材料。在这份报告中，我们表明，从人蜕膜来源的间充质细胞（PCM-DM）制备的细胞周基质是一种有效的基质材料，支持的生长和多能性的hES细胞作为有效的基质胶。PCM-DM的这种支持活性是稳定的，可以在冰箱中保存数月。基于PCM-DM的培养物与非条件商业限定培养基相容，并且与在ROCK抑制剂存在下解离的hES细胞的维持相容。由于蜕膜间充质细胞可以大量制备和扩增，PCM-DM是用于hES细胞临床级培养的动物来源基质的实用的人源性替代物。Developmental Dynamics 238：1118-1130，2009. (C)2009 Wiley-Liss，Inc.
In routine culture, human embryonic stem (hES) cells are maintained on either feeder cells or special culture substrates such as Matrigel. However, to expand hES cells for clinical applications, it is desirable to minimize animal-derived materials in the culture for safety reasons. In this report, we show that the pericellular matrix prepared from human decidua-derived mesenchymal cells (PCM-DM) is a potent substrate material that supports the growth and pluripotency of hES cells as efficiently as Matrigel does. This supporting activity of PCM-DM is stable and can be preserved for several months in the refrigerator. PCM-DM-based culture is compatible with non-conditioned commercial defined medium, and with the maintenance of dissociated hES cells in the presence of ROCK inhibitor. Since decidual mesenchymal cells can be prepared and expanded in a large quantity, PCM-DM is a practical human-derived substitute for the animal-derived substrates for use in clinical-grade culture of hES cells. Developmental Dynamics 238:1118-1130, 2009. (C) 2009 Wiley-Liss, Inc.