Vibrationally resonant imaging of a single living cell by supercontinuum-based multiplex coherent anti-Stokes Raman scattering microspectroscopy
Vibrationally resonant imaging of a single living cell by supercontinuum-based multiplex coherent anti-Stokes Raman scattering microspectroscopy
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DOI：
10.1364/opex.13.001322
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发表时间：
2005-02-21
期刊：
影响因子：
3.8
通讯作者：
Hamaguchi, H
中科院分区：
文献类型：
--
作者：
Kano, H;Hamaguchi, H
基于超连续谱的多重相干反斯托克斯拉曼散射（汽车）显微光谱已被应用于活的裂变酵母细胞的振动成像。我们已经成功地提取了只有一个振动共振汽车图像从一个特征的光谱轮廓中的C-H伸缩振动区域。使用我们简单但灵敏的分析，与固定拉曼位移的汽车成像相比，振动对比度得到显著改善。活酵母细胞的汽车图像显示了信号非常强的几个区域。它们被认为是由线粒体产生的。(C)2005年，美国光学学会。
Supercontinuum-based multiplex coherent anti-Stokes Raman scattering ( CARS) microspectroscopy has been applied to vibrational imaging of a living fission yeast cell. We have successfully extracted only a vibrationally resonant CARS image from a characteristic spectral profile in the C-H stretching vibrational region. Using our simple but sensitive analysis, the vibrational contrast is significantly improved in comparison with a CARS imaging at a fixed Raman shift. The CARS image of a living yeast cell indicates several areas at which the signal is remarkably strong. They are considered to arise from mitochondria. (C) 2005 Optical Society of America.