Interspecific Y chromosome variation is sufficient to rescue hybrid male sterility and is influenced by the grandparental origin of the chromosomes
Interspecific Y chromosome variation is sufficient to rescue hybrid male sterility and is influenced by the grandparental origin of the chromosomes
复制标题
DOI：
10.1038/hdy.2016.11
复制
发表时间：
2016-06-01
期刊：
影响因子：
3.8
通讯作者：
Lemos, B.
中科院分区：
文献类型：
--
作者：
Araripe, L. O.;Tao, Y.;Lemos, B.
Y染色体表现出种内和种间的群体变异。种群内的共同进化有望在Y染色体和基因组的其余部分之间产生适应性的相互作用。其中一个后果是，来自不同群体的Y染色体可能会扰乱共同进化的遗传元素之间的和谐相互作用，并导致男性生育力降低、不育或丧失生存能力。在这里，我们讨论了‘异种Y染色体’对携带纯合区域的毛果果蝇杂交种雄性的生育能力的贡献，该区域导入了仿生果蝇的背景中。为了检测Y染色体与常染色体的相互作用，在常染色体的单一物种背景下可能没有被注意到，我们构建了涉及三个姊妹种的杂交基因型：模拟果蝇、毛果果蝇和果蝇。这些工程菌的差异是由于：(1)Y染色体的物种起源(D·simans或D·sechellia)；(2)在D·Simans第三条染色体上导入的D·mauritiana片段的位置；(3)祖父母基因组背景(D·simans的3个基因类型)。我们发现Y染色体的物种起源、毛滴虫片段的同一性和捐献染色体的祖父母遗传背景之间存在复杂的相互作用。出人意料的是，在Ysim存在的情况下，Y染色体和毛叶石杉的一个片段的相互作用显著降低了育性，而当D.sechellia的Y染色体来自特定的祖父母基因型时，它的育性被部分挽救。尽管有一个常染色体和X连锁的基因组，但生育能力的恢复仍然发生，这主要是源于拟态盘藻。这些结果说明了涉及Y染色体的遗传交互作用的多因素基础。我们的研究支持Y染色体可以显著促进生殖隔离进化的假设，并强调了在特定的基因组合中不孕不育的条件表现。
Y chromosomes display population variation within and between species. Co-evolution within populations is expected to produce adaptive interactions between Y chromosomes and the rest of the genome. One consequence is that Y chromosomes from disparate populations could disrupt harmonious interactions between co-evolved genetic elements and result in reduced male fertility, sterility or inviability. Here we address the contribution of 'heterospecific Y chromosomes' to fertility in hybrid males carrying a homozygous region of Drosophila mauritiana introgressed in the Drosophila simulans background. In order to detect Y chromosome-autosome interactions, which may go unnoticed in a single-species background of autosomes, we constructed hybrid genotypes involving three sister species: Drosophila simulans, D. mauritiana, and D. sechellia. These engineered strains varied due to: (i) species origin of the Y chromosome (D. simulans or D. sechellia); (ii) location of the introgressed D. mauritiana segment on the D. simulans third chromosome, and (iii) grandparental genomic background (three genotypes of D. simulans). We find complex interactions between the species origin of the Y chromosome, the identity of the D. mauritiana segment and the grandparental genetic background donating the chromosomes. Unexpectedly, the interaction of the Y chromosome and one segment of D. mauritiana drastically reduced fertility in the presence of Ysim, whereas the fertility is partially rescued by the Y chromosome of D. sechellia when it descends from a specific grandparental genotype. The restoration of fertility occurs in spite of an autosomal and X-linked genome that is mostly of D. simulans origin. These results illustrate the multifactorial basis of genetic interactions involving the Y chromosome. Our study supports the hypothesis that the Y chromosome can contribute significantly to the evolution of reproductive isolation and highlights the conditional manifestation of infertility in specific genotypic combinations.