Faecal microbiota study reveals specific dysbiosis in spondyloarthritis
Faecal microbiota study reveals specific dysbiosis in spondyloarthritis
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DOI：
10.1136/annrheumdis-2016-211064
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发表时间：
2017-09-01
影响因子：
27.4
通讯作者：
Sokol, Harry
中科院分区：
文献类型：
--
作者：
Breban, Maxime;Tap, Julien;Sokol, Harry
目的 微生物群组成的改变或菌群失调被怀疑与慢性炎症性疾病，如脊柱关节炎 (SpA) 和类风湿性关节炎 (RA) 的发病机制有关。方法 165 对从粪便样本中分离的粪便 DNA 进行了两个连续的横断面队列的核糖体 RNA 基因测序，每个队列由三组成年志愿者组成：SpA、RA 和健康对照 (HC)。在第二项研究中，HC 大部分由已知 HLA-B27 状态的患者的年龄匹配的兄弟姐妹组成。使用 QIIME 评估α和β多样性，并使用线性判别分析效应大小进行比较，以检查组之间的差异。 结果 在两个队列中，与 HC 相比，SpA 和 RA 中均出现菌群失调，并且具有疾病特异性。在两个疾病组中均检测到微生物群生物多样性的限制。最显着的变化是与 RA 和 HC 相比，SpA 中的瘤胃球菌丰度增加了两到三倍，这在两项研究中都很显着，并且与有炎症性肠病 (IBD) 病史的患者的疾病活动度呈正相关。在 HC 中，HLA-B27+ 和 HLA-B27 阴性兄弟姐妹之间的微生物群组成也存在显着差异，这表明遗传背景可能会影响肠道微生物群组成。结论我们的结果表明，SpA 和 RA 都具有独特的生态失调特征，并证明 R. gnavus 出现了可重复的增加，这种增加对 SpA 具有特异性，也是疾病活动的标志物。这一观察结果与该细菌已知的促炎作用及其与 IBD 的关联一致。它可以为 SpA 和 IBD 之间存在的联系提供解释。
Objective Altered microbiota composition or dysbiosis is suspected to be implicated in the pathogenesis of chronic inflammatory diseases, such as spondyloarthritis (SpA) and rheumatoid arthritis (RA).Methods 165 ribosomal RNA gene sequencing was performed on faecal DNA isolated from stool samples in two consecutive cross-sectional cohorts, each comprising three groups of adult volunteers: SpA, RA and healthy controls (HCs). In the second study, HCs comprised a majority of aged-matched siblings of patients with known HLA-B27 status. Alpha and beta diversities were assessed using QIIME, and comparisons were performed using linear discriminant analysis effect size to examine differences between groups.Results In both cohorts, dysbiosis was evidenced in SpA and RA, as compared with HCs, and was disease specific. A restriction of microbiota biodiversity was detected in both disease groups. The most striking change was a twofold to threefold increased abundance of Ruminococcus gnavus in SpA, as compared with both RA and HCs that was significant in both studies and positively correlated with disease activity in patients having a history of inflammatory bowel disease (IBD). Among HCs, significant difference in microbiota composition were also detected between HLA-B27+ and HLA-B27 negative siblings, suggesting that genetic background may influence gut microbiota composition.Conclusion Our results suggest that distinctive dysbiosis characterise both SpA and RA and evidence a reproducible increase in R. gnavus that appears specific for SpA and a marker of disease activity. This observation is consistent with the known proinflammatory role of this bacteria and its association with IBD. It may provide an explanation for the link that exists between SpA and IBD.