Acute effects of testicular and adrenal cortical blockade on protein synthesis and dihydrotestosterone content of human prostate tissue.
Acute effects of testicular and adrenal cortical blockade on protein synthesis and dihydrotestosterone content of human prostate tissue.
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睾丸和肾上腺皮质阻断对人类前列腺组织蛋白质合成和二氢睾酮含量的急性影响。
DOI：
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发表时间：
1985
影响因子：
5.8
通讯作者：
M. Kirshner
中科院分区：
文献类型：
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作者：
Junda Liu;J. Geller;J. Albert;M. Kirshner
为了检测整个前列腺双氢睾酮(DHT)浓度与上皮和间质蛋白质合成之间的关系，对等待良性前列腺肥大手术的患者进行了药物治疗，以降低循环和组织雄激素水平，并与未治疗患者的水平进行比较。这些药物要么是孕激素抗雄激素醋酸甲地孕酮，要么是甲地孕酮加地塞米松，在手术前服用1周。在手术中获得的前列腺组织的等量进行了DHT检测。用酶分离法将剩余的组织分离成间质细胞和上皮细胞，然后测定[~3H]脯氨酸和[~3H]亮氨酸在基质蛋白和上皮细胞蛋白中的掺入。在广泛的组织DHT浓度范围内，DHT与间质蛋白的[~3H]Pro掺入显著相关(r=0.58)。同样，上皮细胞将[~3H]亮氨酸掺入蛋白质与组织DHT浓度显著相关，相关系数为0.79。在基质细胞中，地塞米松和甲地孕酮对基质蛋白合成没有额外的影响。与单独使用甲地孕酮相比，地塞米松和甲地孕酮联合应用能显著提高上皮细胞[~3H]亮氨酸掺入蛋白质的量，尽管组织DHT和循环中的肾上腺雄激素--硫酸脱氢表雄酮和β-雄烯二酮与单独使用甲地孕酮后的结果并无显著差异。这些研究支持这样的结论，即DHT在调节前列腺上皮细胞和间质细胞的蛋白质合成方面起主导作用。地塞米松似乎对前列腺上皮细胞有刺激生长的作用。
To examine the relationship between whole prostatic dihydrotestosterone (DHT) concentrations and epithelial and stromal protein synthesis, patients awaiting surgery for benign prostatic hypertrophy were given medication to reduce circulating and tissue androgen levels for comparison to levels in untreated patients. The medications, either megestrol acetate, a progestational antiandrogen, or megestrol acetate plus dexamethasone, were given for 1 week before surgery. Aliquots of prostate tissue obtained at surgery were assayed for DHT. The remainder of the tissue was separated into stromal and epithelial cells using enzymatic separation; the incorporation of both [3H] proline into stromal protein and [3H]leucine into epithelial cell protein was then measured. Over a wide range of tissue DHT concentrations, DHT correlated significantly with [3H]proline incorporation into stromal protein (r = 0.58). Likewise, epithelial cell incorporation of [3H]leucine into protein correlated significantly with tissue DHT concentrations, with a r value of 0.79. In the case of stromal cells, no additional effects of dexamethasone given with megestrol acetate were found on stromal protein synthesis. Epithelial cell [3H]leucine incorporation into protein was paradoxically enhanced by dexamethasone plus megestrol acetate compared to the latter alone, despite the fact that tissue DHT and circulating adrenal androgens, dehydroepiandrosterone sulfate and delta 4-androstenedione, were not significantly different from values obtained after treatment with megestrol acetate alone. These studies support the conclusion that there is a dominant role of DHT in regulating protein synthesis in both prostatic epithelial and stromal cells. Dexamethasone appears to have a growth-stimulating effect on prostatic epithelial cells.