Preliminary crystallographic studies on human apo-lactoferrin in its native and deglycosylated forms.
Preliminary crystallographic studies on human apo-lactoferrin in its native and deglycosylated forms.
复制标题
对天然形式和去糖基化形式的人脱铁乳铁蛋白的初步晶体学研究。
DOI：
10.1016/0022-2836(89)90283-0
复制
发表时间：
1989
影响因子：
5.6
通讯作者：
Baker,EN
中科院分区：
文献类型：
--
作者：
Norris,GE;Baker,HM;Baker,EN
天然和去糖基化形式的人脱辅基乳铁蛋白已被纯化，并且通过对低离子强度缓冲溶液透析获得晶体。天然脱辅基乳铁蛋白的晶体是斜方晶系，空间群P2 1 2 1 2 1，晶胞尺寸a= 222.0 A/cm，B= 115.6 A/cm，c= 77.8 A/cm，并且每个不对称单元具有两个蛋白质分子。已经获得了两种去糖基化的脱辅基乳铁蛋白的晶体形式。一种是正交晶系，空间群P2 1 2 1 2 1，晶胞尺寸a= 152.1 A，B= 94.6 A，c= 55.8 A。第二个是四元环，空间群I4，晶胞尺寸a= B= 189.4 A，c= 55.1 A。后者的每个不对称单元都只有一个分子，适合于高分辨率的X射线结构分析。
Human apo-lactoferrin in both native and deglycosylated forms has been purified, and crystals obtained by dialysis against low ionic strength buffer solutions. The crystals of native apo-lactoferrin are orthorhombic, space group P2 1 2 1 2 1 with cell dimensions a= 222.0 A ̊, b= 115.6 A ̊, c= 77.8 A ̊ and have two protein molecules per asymmetric unit. Two crystal forms of deglycosylated apo-lactoferrin have been obtained. One is orthorhombic, space group P2 1 2 1 2 1, with cell dimensions a= 152.1 A ̊, b= 94.6 A ̊, c= 55.8 A ̊. The second is tetragonal, space group I4, with cell dimensions a= b= 189.4 A ̊, c= 55.1 A ̊. Both of the latter have only one molecule per asymmetric unit, and are suitable for high-resolution X-ray structure analysis.