A new physical mapping approach refines the sex-determining gene positions on the Silene latifolia Y-chromosome.
A new physical mapping approach refines the sex-determining gene positions on the Silene latifolia Y-chromosome.
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DOI：
10.1038/srep18917
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发表时间：
2016-01-08
影响因子：
4.6
通讯作者：
Kawano S
中科院分区：
文献类型：
--
作者：
Kazama Y;Ishii K;Aonuma W;Ikeda T;Kawamoto H;Koizumi A;Filatov DA;Chibalina M;Bergero R;Charlesworth D;Abe T;Kawano S
对于分子遗传学和进化研究来说，性染色体是基因组中特别有趣的区域;然而，对于大多数物种来说，我们缺乏基本信息，例如沿着染色体的基因顺序。因为它们缺乏重组，Y连锁基因不能被遗传定位，留下物理定位作为确定与X染色体同线性和同源性程度的唯一选择。在这里，我们开发了一种新的和通用的方法删除映射的非重组区域解决“旅行推销员问题”，并评估其精度使用模拟数据集。与现有的辐射杂交方法不同，这种方法允许我们将来自不同实验和来源的联合收割机缺失突变体组合起来。我们将我们的方法应用于雌雄异株植物宽叶蝇子草中一组新产生的缺失突变体，并在其Y染色体图谱上精确定位了性别决定基因座。
Sex chromosomes are particularly interesting regions of the genome for both molecular genetics and evolutionary studies; yet, for most species, we lack basic information, such as the gene order along the chromosome. Because they lack recombination, Y-linked genes cannot be mapped genetically, leaving physical mapping as the only option for establishing the extent of synteny and homology with the X chromosome. Here, we developed a novel and general method for deletion mapping of non-recombining regions by solving “the travelling salesman problem”, and evaluate its accuracy using simulated datasets. Unlike the existing radiation hybrid approach, this method allows us to combine deletion mutants from different experiments and sources. We applied our method to a set of newly generated deletion mutants in the dioecious plant Silene latifolia and refined the locations of the sex-determining loci on its Y chromosome map.