Indocyanine green: Intracellular uptake and phototherapeutic effects in vitro
Indocyanine green: Intracellular uptake and phototherapeutic effects in vitro
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DOI：
10.1016/s1011-1344(96)07453-2
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发表时间：
1997-04-01
影响因子：
5.4
通讯作者：
Landthaler, M
中科院分区：
文献类型：
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作者：
Fickweiler, S;Szeimies, RM;Landthaler, M
吲哚菁绿色（ICG;在人血浆中的吸收峰805 nm）被研究用于ICG介导的体外光疗。24小时孵育期后，HaCaT角质形成细胞对ICG（1 μ M-50 μ M）的细胞摄取增加至细胞内ICG浓度为12.1 +/- 1.3 nmol/10（6）个细胞。为了检测体外剂量依赖性光毒性效应，将角质形成细胞与0 μ M-50 μ M ICG孵育24小时，并用不同能量密度（0、12、24、48 J cm（-2））的二极管激光（805 nm）照射。除了5 μ M外，所有应用的ICG浓度与辐射组合产生细胞杀伤作用，这显著取决于ICC浓度和光剂量。黑暗对照和用50 μ M ICG孵育并用48 J cm（-2）照射的细胞的细胞活力分别为0.82 +/- 0.15和0.07 +/- 0.02。叠氮化钠（100 mM），一种活性氧猝灭剂，使用50 μ M ICG和24 J cm（-2）可显著抑制细胞杀伤。总之，通过用二极管激光照射ICG的光活化显示出有效诱导HaCaT角质形成细胞的细胞杀伤。这种效应可被叠氮化钠抑制，因此ICG的照射可能会引起光动力学反应。
Indocyanine green (ICG; absorption peak in human plasma 805 nm) was investigated for ICG-mediated phototherapy in vitro. The cellular uptake of ICG (1 mu M-50 mu M) into HaCaT keratinocytes after an incubation period of 24 h increased up to an intracellular ICG concentration of 12.1 +/- 1.3 nmol per 10(6) cells. To examine dose dependent phototoxic effects in vitro, keratinocytes were incubated with 0 mu M-50 mu M ICG for 24 h and irradiated by a diode laser (805 nm) with different energy densities (0, 12, 24, 48 J cm(-2)). All applied ICG concentrations except for 5 mu M yielded a cell killing effect in combination with irradiation depending significantly on ICC concentration and Light dose. Cell viability for dark control and cells incubated with 50 mu M ICG and irradiated with 48 J cm(-2) was 0.82 +/- 0.15 and 0.07 +/- 0.02, respectively. Sodium azide (100 mM), a quencher of reactive oxygen species, inhibited significantly the cell killing using 50 mu M ICG and 24 J cm(-2). Taken together, photoactivation of ICG by irradiation with a diode laser was shown to induce effectively cell killing of HaCaT keratinocytes. Moreover, this effect was inhibited by sodium azide, thus irradiation of ICG might induce a photodynamic reaction.