Characterization and fibrinolytic properties of human omental tissue mesothelial cells. Comparison with endothelial cells.
Characterization and fibrinolytic properties of human omental tissue mesothelial cells. Comparison with endothelial cells.
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人网膜组织间皮细胞的表征和纤溶特性。
DOI：
10.1182/blood.v75.7.1490.bloodjournal7571490
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发表时间：
1990
期刊：
影响因子：
20.3
通讯作者：
van Muijen
中科院分区：
文献类型：
--
作者：
V. Hinsbergh;T. Kooistra;M. A. Scheffer;J.;Hajo van;van Muijen
据报道，网膜脂肪组织是人体微血管内皮细胞的良好来源。通过表征，我们证明从大网膜组织分离的上皮细胞不是内皮细胞，而是间皮细胞。它们含有丰富的细胞角蛋白8和18，这在内皮细胞和静脉蛋白中是不存在的。内皮特异性抗体EN-4和PAL-E未见染色。间皮细胞可见模糊的弥漫性血管性血变因子（vWF）染色，而皮下脂肪微血管内皮细胞显示明显颗粒状结构的vWF。人腹膜间皮产生依赖纤溶酶原激活剂的纤溶活性，这在纤维渗出物的溶解中是必不可少的，因此可能对防止纤维性腹膜粘连的形成很重要。这种纤溶活性依赖于纤溶酶原激活剂，但尚未完全表征。我们在此报道，体外培养的人大网膜组织间皮细胞产生大量的组织型纤溶酶原激活剂（t-PA），以及1型和2型纤溶酶原激活剂抑制剂（PAI-1和PAI-2）。PAI-1主要分泌到培养基中，而PAI-2的大部分在细胞中发现。未检出尿激酶型纤溶酶原激活剂。在炎症介质肿瘤坏死因子（TNF）的刺激下，观察到t-PA抗原至少减少三倍，同时PAI-1和PAI-2增加。TNF也诱导细胞形状的显著变化。尽管TNF和细菌脂多糖（LPS）对人内皮细胞产生PA抑制剂有相似的影响，但LPS对间皮细胞的纤溶特性没有或只有相对较小的影响。细胞因子TNF诱导的纤维蛋白溶解活性降低可能在炎症反应存在时损害腹膜纤维蛋白沉积物的自然溶解。
It has been reported that omental fat tissue is a good source of human microvascular endothelial cells. By characterization we demonstrate that the epitheloid cells isolated from omental tissue are not endothelial cells, but mesothelial cells. They contain abundant cytokeratins 8 and 18, which are absent in endothelial cells, and vimentin. No staining with the endothelial-specific antibodies EN-4 and PAL-E is observed. A faint and diffuse staining of von Willebrand factor (vWF) is seen in mesothelial cells, whereas microvascular endothelial cells from subcutaneous fat display vWF in distinct granular structures. Human peritoneal mesothelium produces plasminogen activator-dependent fibrinolytic activity, which is essential in the resolution of fibrous exudates and may therefore be important in preventing the formation of fibrous peritoneal adhesions. This fibrinolytic activity is plasminogen activator-dependent, but has not been fully characterized. We report here that human omental tissue mesothelial cells in vitro produce large amounts of tissue-type plasminogen activator (t-PA), together with type 1 and 2 plasminogen activator inhibitor (PAI-1 and PAI-2). PAI-1 is predominantly secreted into the culture medium, whereas the major part of PAI-2 is found in the cells. No urokinase-type plasminogen activator is detected. On stimulation with the inflammatory mediator tumor necrosis factor (TNF), at least a threefold decrease in t-PA antigen is observed, together with an increase in both PAI-1 and PAI-2. TNF also induces a marked change in cell shape. Whereas TNF and bacterial lipopolysaccharide (LPS) have similar effects on the production of PA inhibitor by human endothelial cells, LPS has no or only a relatively small effect on the fibrinolytic properties of mesothelial cells. The decreased fibrinolytic activity induced by the cytokine TNF may impair the natural dissolution of fibrin deposits at the peritoneum in the presence of an inflammatory reaction.
人脐带动脉内皮细胞的增殖和形态表型。
影响因子：
6.6
作者：
vanHinsbergh,VW;Mommaas-Kienhuis,AM;Weinstein,R;Maciag,T
通讯作者：
Maciag,T