EFFECT OF TRANSPOSON-INDUCED MOTILITY MUTATIONS ON COLONIZATION OF THE HOST LIGHT ORGAN BY VIBRIO-FISCHERI
EFFECT OF TRANSPOSON-INDUCED MOTILITY MUTATIONS ON COLONIZATION OF THE HOST LIGHT ORGAN BY VIBRIO-FISCHERI
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DOI：
10.1128/jb.176.22.6986-6991.1994
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发表时间：
1994-11-01
影响因子：
3.2
通讯作者：
RUBY, EG
中科院分区：
文献类型：
--
作者：
GRAF, J;DUNLAP, PV;RUBY, EG
费氏弧菌是一种在海水中自由生活的细菌，也是几种鱼类和鱿鱼的特殊的、互惠的光器官共生体。为了确定具有共生能力的菌株成功定植幼体光器官所需的特征，我们利用转座子Mu dI 1681建立了一个突变体池，并对这个突变体池中不再活动的菌株进行筛选。获得了18个独立分离的鞭毛或非鞭毛的非运动突变体。与亲本菌株相比，这些无运动的突变体都不能在幼乌贼的光器官上定居。有鞭毛的非运动性突变菌株NM200具有一束与野生型菌株难以区分的有鞘的极性鞭毛，这表明鞭毛本身的存在不足以定植，成功的轻器官定植需要运动性本身。本研究确定运动性是费氏弧菌的第一个需要的共生表型。
Vibrio fischeri is found both as a free-living bacterium in seawater and as the specific, mutualistic light organ symbiont of several fish and squid species. To identify those characteristics of symbiosis-competent strains that are required for successful colonization of the nascent light organ of juvenile Euprymna scolopes squids, we generated a mutant pool by using the transposon Mu dI 1681 and screened this pool for strains that were no longer motile. Eighteen independently isolated nonmotile mutants that were either flagellated or nonflagellated were obtained. In contrast to the parent strain, none of these nonmotile mutants was able to colonize the juvenile squid light organ. The flagellated nonmotile mutant strain NM200 possessed a bundle of sheathed polar flagella indistinguishable from that of the wild-type strain, indicating that the presence of flagella alone is not sufficient for colonization and that it is motility itself that is required for successful light organ colonization. This study identifies motility as the first required symbiotic phenotype of V. fischeri.