Reversible reduction in dendritic spines in ca1 of rat and ground squirrel subjected to hypothermia-normothermia in vivo:: A three-dimensional electron microscope study
Reversible reduction in dendritic spines in ca1 of rat and ground squirrel subjected to hypothermia-normothermia in vivo:: A three-dimensional electron microscope study
复制标题
DOI：
10.1016/j.neuroscience.2007.07.059
复制
发表时间：
2007-11-09
期刊：
影响因子：
3.3
通讯作者：
Stewart, M. G.
中科院分区：
文献类型：
--
作者：
Popov, V. I.;Medvedev, N. I.;Stewart, M. G.
在电子显微镜水平上研究了地松鼠在低温冬眠（脑温2-4 ℃）和升温恢复至常温（34 ℃）后海马CA 1区树突棘和突触后密度（PSD）的三维形态变化。此外，在非冬眠的哺乳动物大鼠中测量PSD和棘的形态，所述非冬眠的哺乳动物大鼠经受2 ℃的冷却，此时核心直肠温度为15 ℃，然后在升温至正常体温条件后。结果表明，大鼠和地松鼠在常温下与降温或冬眠时相比，其CA 1区薄棘、短棘和轴突触的比例有显著差异。低温诱导细刺比例减少，而蘑菇刺和轴刺比例增加，但蘑菇刺比例没有变化。地松鼠这三类棘的再分布变化比大鼠明显。有没有显着差异的突触密度测定地面松鼠或大鼠在正常与低温相比。棘和PSD体积（蘑菇和薄棘）的测量也表明，无论是大鼠或地松鼠的两种功能状态之间没有显着差异，也没有相邻突触之间的距离有任何差异。树突轴上的小刺是显着的质量在冬眠期间，但在常温缺席。这些数据表明，低温导致的形态学变化在冬眠和非冬眠动物中基本相似。(C)2007年IBRO。由爱思唯尔有限公司出版。保留所有权利。
A study was made at electron microscope level of changes in the three-dimensional (3-D) morphology of dendritic spines and postsynaptic densities (PSDs) in CA1 of the hippocampus in ground squirrels, taken either at low temperature during hibernation (brain temperature 2-4 degrees C), or after warming and recovery to the normothermic state (34 degrees C). In addition, the morphology of PSDs and spines was measured in a non-hibernating mammal, rat, subjected to cooling at 2 degrees C at which time core rectal temperature was 15 degrees C, and then after warming to normothermic conditions. Significant differences were found in the proportion of thin and stubby spines, and shaft synapses in CA1 for rats and ground squirrels for normothermia compared with cooling or hibernation. Hypothermia induced a decrease in the proportion of thin spines, and an increase in stubby and shaft spines, but no change in the proportion of mushroom spines. The changes in redistribution of these three categories of spines in ground squirrel are more prominent than in rat. There were no significant differences in synapse density determined for ground squirrels or rats at normal compared with low temperature. Measurement of spine and PSD volume (for mushroom and thin spines) also showed no significant differences between the two functional states in either rats or ground squirrels, nor were there any differences in distances between neighboring synapses. Spinules on dendritic shafts were notable qualitatively during hibernation, but absent in normothermia. These data show that hypothermia results in morphological changes which are essentially similar in both a hibernating and a non-hibernating animal. (C) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.