Lowest Detectable Protein Immobilization Measurement Using an Ultrasensitive Micropillar-Based Quartz Crystal Microbalance (QCM-P) Device
Lowest Detectable Protein Immobilization Measurement Using an Ultrasensitive Micropillar-Based Quartz Crystal Microbalance (QCM-P) Device
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DOI：
10.1109/jsen.2019.2916102
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发表时间：
2019-11-01
影响因子：
4.3
通讯作者：
Sun, Hongwei
中科院分区：
文献类型：
--
作者：
Esmaeilzadeh, Hamed;Su, Junwei;Sun, Hongwei
开发新的传感技术，以达到最低的检测限的分析物是至关重要的临床测量，如药物测试，肿瘤指标，促甲状腺激素和类似的免疫分析检查。有报道称，将聚合物微柱阵列附加到石英晶体微天平衬底（QCM- p）上形成一个两自由度振动系统，能够显著提高传统QCM系统的灵敏度。本文着重建立了QCM-P器件灵敏度测定的理论模型。为了验证该模型，利用市售QCM平台qCell T操作QCM- p装置，并测量了不同浓度牛血清白蛋白（BSA）的吸附量。灵敏度比传统的基于薄膜的QCM （QCM- f）提高了5倍。此外，QCM-P能够在非常低的浓度下检测BSA蛋白，接近0.3 μ g/mL。
Development of new sensing techniques to achieve the lowest detection limit of analyte is vital for clinical measurements, such as drug testing, tumor indicators, thyroid-stimulating hormone, and similar immunoassay examinations. It has been reported that a polymer micropillar array appended to a quartz crystal microbalance substrate (QCM-P) forms a two-degree-of-freedom vibration system that was able to significantly improve the sensitivity of conventional QCM systems. This paper focuses on the development of a theoretical model for sensitivity determination of the QCM-P devices. To validate the model, a commercially available QCM platform, qCell T, was utilized to operate the QCM-P device and the adsorption of bovine serum albumin (BSA) protein with varied concentrations was measured with the system. A five-fold enhancement in sensitivity over a traditional film-based QCM (QCM-F) was demonstrated. In addition, it shows that the QCM-P had the capability to detect the BSA protein at very low concentrations approaching 0.3 mu g/mL.