THz time domain spectroscopy of biomolecular conformational modes
THz time domain spectroscopy of biomolecular conformational modes
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DOI：
10.1088/0031-9155/47/21/318
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发表时间：
2002-11-07
影响因子：
3.5
通讯作者：
Birge, R
中科院分区：
文献类型：
--
作者：
Markelz, A;Whitmire, S;Birge, R
我们讨论了使用太赫兹时域光谱的构象灵活性和生物分子的构象变化的研究。蛋白质结构动力学对生物功能至关重要，蛋白质柔性影响酶促反应速率和感觉转导循环时间。构象模式动力学发生在皮秒时间尺度上，并且集体振动模式与1-100 cm（-1）范围内的这些大尺度结构运动相关。我们已经进行了太赫兹时域光谱（TTDS）的几个生物分子系统，以探讨TTDS的灵敏度，以区分不同的分子种类，不同的突变在一个单一的物种和不同的构象的一个给定的生物分子。我们比较了测量到的TTDS的正常模式的计算，发现TTDS吸光度反映了正常模式的分子力学计算确定的密度，是敏感的构象和突变。这些早期的研究证明了使用TTDS研究生物分子的一些优点和局限性。
We discuss the use of terahertz time domain spectroscopy for studies of conformational flexibility and conformational change in biomolecules. Protein structural dynamics are vital to biological function with protein flexibility affecting enzymatic reaction rates and sensory transduction cycling times. Conformational mode dynamics occur on the picosecond timescale and with the collective vibrational modes associated with these large scale structural motions in the 1-100 cm(-1) range. We have performed THz time domain spectroscopy (TTDS) of several biomolecular systems to explore the sensitivity of TTDS to distinguish different molecular species, different Mutations within a single species and different conformations of a given biomolecule. We compare the measured absorbances to normal mode calculations and find that the TTDS absorbance reflects the density of normal modes determined by molecular mechanics calculations, and is sensitive to both conformation and mutation. These early studies demonstrate some of the advantages and limitations of using TTDS for the study of biomolecules.