Fast Stiffness Mapping of Cells Using High-Bandwidth Atomic Force Microscopy.
Fast Stiffness Mapping of Cells Using High-Bandwidth Atomic Force Microscopy.
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DOI：
10.1021/acsnano.5b03959
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发表时间：
2016-01-26
期刊：
影响因子：
17.1
通讯作者：
Butte MJ
中科院分区：
文献类型：
--
作者：
Wang A;Vijayraghavan K;Solgaard O;Butte MJ
The cytoskeleton controls cellular morphology and mediates the mechanical interactions between a cell and its environment. Atomic force microscopy (AFM) has the unique capability to map cytoskeletal mechanics and structures with nanometer resolution. However, whole–cell cytomechanical imaging with conventional AFM techniques is limited by low imaging speed. Here we present fast nanomechanical mapping of cells using high–bandwidth AFM (HB–AFM), where >106 nanoindentation measurements were acquired in ~10 min—a task that would take weeks to finish using conventional AFM. High bandwidth measurements enabled capture of the entire tip–sample interaction for each tap on cells, engendering a new measurement (“force phase”) that exceeds the contrast of conventional tapping mode, and enabling spectral visualization of >10 harmonics. The abundance of measurements allowed discovery of subtle cytomechanical features, including the stiffness of fibers of the cellular spectrin network in situ. This approach bridges HB–AFM and high–harmonic imaging, and opens future opportunity towards dynamic mechanical properties of living cells.
