A 62-kDa mitotic apparatus protein required for mitotic progression is sequestered to the interphase nucleus by associating with the chromosomes during anaphase.
A 62-kDa mitotic apparatus protein required for mitotic progression is sequestered to the interphase nucleus by associating with the chromosomes during anaphase.
复制标题
有丝分裂进展所需的 62 kDa 有丝分裂装置蛋白通过在后期与染色体结合而被隔离到间期核。
DOI：
10.1002/cm.970300408
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Sloboda,RD
中科院分区：
文献类型：
--
作者：
Ye,X;Sloboda,RD
海胆胚胎有丝分裂器中的62-kDa（p62）蛋白质组分已被证明对有丝分裂的正常进行很重要[Dinsmore和斯洛博达，1989：Cell 57：127-134]。为了研究p62在海胆胚胎细胞周期中的亚细胞分布，采用间接免疫荧光显微镜结合改良的去污剂提取程序。通过这种方法获得的改进的荧光图像提供了关于细胞周期中p62的亚细胞定位的新信息，这是用以前的常规染色方法不能获得的[约翰斯顿和斯洛博达，1992：J. Cell Biol.119：843-854]。使用p62的亲和纯化抗体，我们观察到p62在染色体/染色质上的细胞周期依赖性定位。在第一或第二细胞周期的核膜破裂之前，p62定位于细胞核中的染色质。在有丝分裂期间，p62与有丝分裂器的微管所占据的纺锤体区域相关联。随着分裂后期的进行，但在核膜重组之前，p62逐渐与染色体结合。因此，p62被纳入形成间期核由于其与染色体在后期，而不是主动易位到新形成的子核通过核孔。在这些细胞中，p62的定位与棘皮动物胚胎中的蛋白定位相似，这表明它可能在哺乳动物体细胞的有丝分裂过程中发挥作用。© 1995 Wiley利斯公司
A protein component of 62‐kDa (p62) in the mitotic apparatus of the sea urchin embryo has been shown to be important for the proper progression of mitosis [Dinsmore and Sloboda, 1989:Cell 57:127–134]. To study the subcellular distribution of p62 during the cell cycle of sea urchin embryos, indirect immunofluorescence microscopy was used coupled to a modified detergent extraction procedure. The improved fluorescent images obtained by this procedure provide new information concerning the subcellular localization of p62 during the cell cycle that could not be obtained with previous conventional staining procedures [Johnston and Sloboda, 1992:J. Cell Biol.119:843–854]. Using affinity purified antibodies to p62, we observed a cell cycle‐dependent localization of p62 to the chromosomes/chromatin. Prior to nuclear envelope breakdown of the first or second cell cycle, p62 localizes to chromatin in the nucleus. During mitosis, p62 associates with the region of the spindle occupied by the microtubules of the mitotic apparatus. As anaphase proceeds, but before the nuclear envelope reforms, p62 becomes progressively associated with the chromosomes. Thus, p62 is incorporated into the forming interphase nucleus due to its association with chromosomes during late anaphase, rather than by active translocation into the newly formed daughter nuclei through the nuclear pores. The protein is not unique to marine embryos, as demonstrated by immunofluorescence of Y‐1 cells, a mouse adrenal tumor cell line In these cells, the localization of p62 is similar to the localization of the protein in echinoderm embryos, suggesting its possible function in mitotic progression in mammalian somatic cells as well. © 1995 Wiley‐Liss, Inc.