Physiological role of germicidins in spore germination and hyphal elongation in Streptomyces coelicolor A3(2)
Physiological role of germicidins in spore germination and hyphal elongation in Streptomyces coelicolor A3(2)
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DOI：
10.1038/ja.2011.59
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发表时间：
2011-09-01
影响因子：
3.3
通讯作者：
Natsume, Masahiro
中科院分区：
文献类型：
--
作者：
Aoki, Yuu;Matsumoto, Daisuke;Natsume, Masahiro
从天蓝色链霉菌A3的液体培养物中分离到四种杀菌素同系物（2）。这些被鉴定为杀菌素A、B和C，以及苏鲁卡比龙A（杀菌素D）。确定杀菌素A和C的手性中心的绝对立体化学为S。所有杀菌素均抑制S.天蓝色A3（2）孢子大于1 μ g ml（-1）。S.从单个培养皿（9 cm i.d.）含杀菌素A 5.4 μ g（相当于2.7 × 10 ~（-14）g/孢子），占孢子提取物的2.3%，杀菌素B、C、D含量为0.2-0.8 μ g。孢子提取物的活性与各杀菌素的活性之和具有良好的对应关系，表明杀菌素在S. coelicolor A3（2）.杀菌素A对孢子萌发的抑制作用是可逆的，杀菌素A不仅抑制孢子萌发，而且抑制菌丝伸长。The Journal of Antibiotics（2011）64，607-611; doi：10.1038/ja.2011.59; 2011年7月27日在线发表
Four germicidin homologs were isolated from a liquid culture of Streptomyces coelicolor A3(2). These were identified as germicidins A, B and C, and surugapyrone A (germicidin D). Absolute stereochemistry of the chiral center in germicidins A and C is determined to be S. All germicidins inhibited germination of S. coelicolor A3(2) spores above 1 mu g ml(-1). S. coelicolor A3(2) spores collected from a single petri dish (9cm i.d.) contained 5.4 mu g of germicidin A (similar to 2.7 x 10(-14) g per spore), which accounts for 2.3% of the spore extract, and contents of germicidins B, C and D were 0.2-0.8 mu g. The activity of the spore extract corresponded well with the sum of the activity of each germicidin, which was estimated from the content and dose-response curve, which indicates that germicidins functions as self-germination inhibitors in S. coelicolor A3(2). Inhibitory action of germicidin A on spore germination was reversible and germicidin A inhibited not only spore germination but also hyphal elongation. The Journal of Antibiotics (2011) 64, 607-611; doi: 10.1038/ja.2011.59; published online 27 July 2011