Use of liquid chromatography-mass spectrometry for the quantitation of dethiobiotin and biotin in biological samples.
Use of liquid chromatography-mass spectrometry for the quantitation of dethiobiotin and biotin in biological samples.
复制标题
使用液相色谱-质谱法定量生物样品中的脱硫生物素和生物素。
DOI：
10.1016/s0021-9673(01)96076-5
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Y. Georges
中科院分区：
文献类型：
--
作者：
M. Azoulay;P. Desbène;F. Frappier;Y. Georges
这是通过使用Izumi等人所描述的Bacilhres sphaericus进行的。18.培养后收获细胞，上清液在减压下蒸发至干燥。用99%乙醇提取DTB和生物素，并通过Dowex AG L-X2甲酸酯柱层析纯化，如上所述lgWzl。用新鲜制备的重氮甲烷从酸中得到甲酯，在硅胶60柱(70-200目)上，以乙酸乙酯-甲醇(9：L)为洗脱液进行层析。收集与DTB和生物素甲酯对应的馏分(与标准柱相比)，在真空中浓缩，用于LC-MS研究。
This was performed by using Bacilhrs sphaericus as described by Izumi et al. 18. After incubation, the cells were harvested and the supernatant evaporated to dryness under reduced pressure. The DTB and biotin were extracted with 99% ethanol and purified by column chromatography on a Dowex AG l-X2 formate column as previously described lgWzl. The methyl esters were obtained from the acid using freshly prepared diazomethane and chromatographed on a silica gel 60 column (70-200 mesh) using ethyl acetate-methanol (9: l) as eluent. The fractions corresponding to the DTB and biotin methyl esters (compared to standard column) were collected, concentrated in vacua and used for LC-MS studies.