Molecular epidemiological analysis of bat rabies viruses in Brazil
Molecular epidemiological analysis of bat rabies viruses in Brazil
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DOI：
10.1292/jvms.67.647
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发表时间：
2005-07-01
影响因子：
1.2
通讯作者：
Sakai, T
中科院分区：
文献类型：
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作者：
Kobayashi, Y;Sato, G;Sakai, T
在巴西圣保罗，对从吸血、食果和食虫蝙蝠中分离的19种狂犬病病毒（RV）进行了分子流行病学分析。采用RT-PCR方法扩增RV核蛋白（N）基因，直接测序法测定N基因1,335个核苷酸序列。系统发育分析，这是基于目前和以前确定的巴西RV分离株的N基因，揭示了从蝙蝠中分离的RV在遗传上分为四个谱系，有依赖于宿主蝙蝠物种的倾向。第一个谱系主要由吸血蝙蝠（Desmodus rotundus）分离株组成，包括食果蝙蝠（Artibeus spp.）分离株其他三个谱系由食虫蝙蝠分离物组成;主要是Eptesicus spp.，松藻属和夜猫子属（Nyctinomops spp.）分离，分别。这些结果表明，有蝙蝠物种特异性RV变种在巴西的可能性。
A molecular epidemiological analysis was performed in 19 rabies viruses (RVs) isolated from haematophagous, frugivorous and insectivorous bats, in Sao Paulo, Brazil. The authors carried out RT-PCR for amplification of the RV nucleoprotein (N) gene, and determined 1,335 nucleotide sequences of N gene by direct sequencing method. Phylogenetic analysis, which was based on the N gene of Brazilian RV isolates identified presently and previously, revealed that RVs isolated from bats were genetically divided into four lineages had a tendency to depend on the host bat species. The first lineage consisted mainly of haematophagous bat (Desmodus rotundus) isolates, including frugivorous bat (Artibeus spp.) isolates. Other three lineages consisted of insectivorous bat isolates; mainly Eptesicus spp., Molossus spp. and Nyctinomops spp. isolates, respectively. These results indicate a possibility of that there are bat species-specific RV variants in Brazil.