A Sericin-Derived Peptide Protects Sf9 Insect Cells from Death Caused by Acute Serum Deprivation
A Sericin-Derived Peptide Protects Sf9 Insect Cells from Death Caused by Acute Serum Deprivation
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DOI：
10.1007/s10529-005-7177-5
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发表时间：
2005-07
影响因子：
2.7
通讯作者：
Masakazu Takahashi;K. Tsujimoto;Youichi Kato;H. Yamada;H. Takagi;S. Nakamori
中科院分区：
文献类型：
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作者：
Masakazu Takahashi;K. Tsujimoto;Youichi Kato;H. Yamada;H. Takagi;S. Nakamori
丝胶是蚕茧中包裹丝素纤维的丝蛋白。丝胶水解物保护培养的Sf9昆虫细胞免受血清剥夺所致的死亡；其活性依赖于38个氨基酸的重复。来自38个残基的部分肽SGGSSTYGYS也可以抑制血清剥夺死亡。接种后第3天，含10%(v/v)胎牛血清、无添加剂和1mMSGGSSTYGYS的细胞存活率分别为96%、12%和31%。芳香族残基似乎很重要，因为SGGSSTWGWS具有与SGGSSTYGYS相同的活性，而SGGSSTAGAS没有活性。
Sericin is the silk protein enveloping fibroin fibers in cocoons. Sericin hydrolysate protects cultured Sf9 insect cells from death caused by serum deprivation; the activity depends on the repeats of 38 amino acids. A partial peptide from the 38 residues, SGGSSTYGYS, inhibited serum-deprivation death as well. Cell viabilities in the presence of 10% (v/v) foetal calf serum, no additives and 1 mMSGGSSTYGYS were 96, 12 and 31% on the third day after inoculation, respectively. Aromatic residues seemed to be important because SGGSSTWGWS had the same activity as SGGSSTYGYS but SGGSSTAGAS had no activity.