PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus
PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus
复制标题
DOI：
10.1016/j.nbt.2010.04.003
复制
发表时间：
2010-09-30
影响因子：
5.4
通讯作者：
Ishida, Betty K.
中科院分区：
文献类型：
--
作者：
Bartley, Glenn E.;Breksa, Andrew P., III;Ishida, Betty K.
背景酚胺缩酮是一种血管收缩剂和支气管扩张剂，有望成为体重管理和减肥的辅助药物。Synetamine在结构上类似于几种商业感冒药的活性成分。一些柑橘含有高浓度的辛烯酮。酪氨酸脱羧酶（TYDC）是一种参与合成酪氨酸生物合成的酶，是一种吡哆醛-5 '-磷酸（PLP）依赖性酶，可使酪氨酸脱羧产生CO2和酪胺。我们用PCR来筛选，从不同的柑橘属物种和品种中克隆并测序了该基因，以确定该基因的DNA序列是否与柑橘属植物中的聚乙烯醇的存在相关。结果PCR扩增和DNA序列比较表明，DNA序列差异可能导致一些不产生聚乙烯醇的柑橘产生截短蛋白。结论聚乙烯醇的产生可能部分由遗传因素决定，TYDC基因。
BackgroundThe phenolic amine synephrine is a vascoconstrictor and bronchiectatic agent and holds promise as an aid to weight management and obesity reduction. Synephrine is structurally similar to the active ingredients of several commercial cold remedies. Some Citrus contain high concentrations of synephrine. An enzyme involved in synephrine biosynthesis, tyrosine decarboxylase (TYDC), is a pyridoxal-5'-phosphate (PLP)-dependent enzyme that decarboxylates tyrosine to yield CO2 and tyramine. We used PCR to screen, clone and sequence this gene from various synephrine producing and nonproducing Citrus species and varieties to determine if DNA sequence of this gene correlated with synephrine presence.ResultsPCR amplification and comparison of DNA sequence indicates DNA sequence differences that may cause production of truncated proteins to be produced in some nonsynephrine producing Citrus.ConclusionSynephrine production may be genetically determined in part by the gene for TYDC.