Prevascularisation with endothelial progenitor cells improved restoration of the architectural and functional properties of newly formed bone for bone reconstruction
Prevascularisation with endothelial progenitor cells improved restoration of the architectural and functional properties of newly formed bone for bone reconstruction
复制标题
内皮祖细胞预血管化改善了新形成骨的结构和功能特性的恢复，用于骨重建
DOI：
10.1007/s00264-012-1751-y
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发表时间：
2013-04-01
影响因子：
2.7
通讯作者：
Xu, Jian-Zhong
中科院分区：
文献类型：
--
作者：
Pang, Hao;Wu, Xue-Hui;Xu, Jian-Zhong
本研究的目的是探讨内皮祖细胞的加入是否有助于修复新形成的骨的结构和功能特性，用于骨缺损的修复。方法将骨髓来源的内皮祖细胞和间充质干细胞共同种植于脱钙骨基质(DBM)上，作为预血运组织工程骨(TEB)修复节段性骨缺损，评价预血运组织工程骨对骨形态、血流动力学和力学性能的影响。在骨重建过程中，血运重建前组的血供、生物力学强度和骨密度均显著高于未血运重建组。结论EPC依赖的血运重建促进骨愈合，促进结构重建和功能恢复，有助于加深对血管生成和成骨之间关系的认识。
PurposeThe aim of this study was to examine whether the addition of endothelial progenitor cells (EPCs) contributes to restoring the architectural and functional properties of newly formed bone for reconstruction of bone defects.MethodsBone marrow-derived EPCs and mesenchymal stem cells (MSCs) were co-seeded onto demineralized bone matrix (DBM) as a prevascularized tissue-engineered bone (TEB) for the repair of segmental bone defects to evaluate the effects of prevascularization of TEB on ameliorating morphological, haemodynamic and mechanical characteristics.ResultsThe restoration of the intraosseous vasculature and medullary cavity was improved markedly compared to the non-prevascularized groups. The blood supply, biomechanical strength, and bone mineral density of the prevascularized group were significantly higher than those of the non-prevascularized groups during bone reconstruction.ConclusionsThe present study indicates that EPC-dependent prevascularization contributes to bone healing with structural reconstruction and functional recovery and may improve the understanding of correlation between angiogenesis and osteogenesis.