Yeast artificial chromosome segregation from host chromosomes with similar lengths.
Yeast artificial chromosome segregation from host chromosomes with similar lengths.
复制标题
酵母人工染色体与长度相似的宿主染色体分离。
DOI：
10.1093/nar/26.21.5011
复制
发表时间：
1998
影响因子：
14.9
通讯作者：
Frank-Kamenetskii,MD
中科院分区：
文献类型：
--
作者：
Izvolsky,KI;Demidov,VV;Bukanov,NO;Frank-Kamenetskii,MD
We propose a new method for segregation of yeast artificial chromosomes (YACs) from endogenous yeast chromosomes with similar lengths. The method is based on recently developed PNA-assisted rare cleavage (PARC) of genomic DNA. We apply the PARC procedure to YAC-containing samples of yeast DNA in such a way that host chromosomes, which electrophoretically comigrate with the chosen YACs, are selectively digested while YACs remain intact. These data demonstrate that a pool of appropriate PNAs can be used as an efficient tool for the PARC-based isolation of intact purified YACs directly from the host cells.
