Targeting Tumor Cells by Natural Anti-Carbohydrate Antibodies Using Rhamnose-Functionalized Liposomes
Targeting Tumor Cells by Natural Anti-Carbohydrate Antibodies Using Rhamnose-Functionalized Liposomes
复制标题
使用鼠李糖功能化脂质体通过天然抗碳水化合物抗体靶向肿瘤细胞
DOI：
10.1021/acschembio.6b00173
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发表时间：
2016-05-01
影响因子：
4
通讯作者：
Yi, Wen
中科院分区：
文献类型：
--
作者：
Li, Xuexia;Rao, Xiongjian;Yi, Wen
在人类免疫系统中募集抗体用于靶向破坏肿瘤细胞已成为令人兴奋的研究领域，这是由于其副作用发生率低、功效高和特异性高。人血清中存在大量的抗碳水化合物天然抗体，这促使人们努力利用碳水化合物表位进行免疫募集。在这里，我们已经开发了一种基于鼠李糖功能化脂质体的肿瘤细胞特异性靶向破坏的一般策略。用鼠李糖表位人工修饰的肿瘤细胞在体外经受补体介导的细胞毒性，并且在体内显示出延迟的肿瘤生长。这项研究强调了通过细胞表面聚糖工程激活内源性免疫应答的治疗潜力。
Recruitment of antibodies in human immune systems for targeted destruction of tumor cells has emerged as an exciting area of research due to its low occurrence of side effects, high efficacy, and high specificity. The presence of large amounts of anticarbohydrate natural antibodies in human sera has prompted research efforts to utilize carbohydrate epitopes for immune recruitment. Here, we have developed a general strategy for specific targeted destruction of tumor cells based on rhamnose-functionalized liposomes. Tumor cells artificially decorated with rhamnose epitopes were subjected to complement mediated cytotoxicity in vitro and showed delayed tumor growth in vivo. This study highlights the therapeutic potential for activation of endogenous immune response through cell-surface glycan engineering.