Reprogramming somatic gene activity by fusion with pluripotent cells
Reprogramming somatic gene activity by fusion with pluripotent cells
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DOI：
10.1007/bf02698052
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发表时间：
2006-01-01
期刊：
影响因子：
--
通讯作者：
Schoeler, Hans R.
中科院分区：
文献类型：
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作者：
Do, Jeong Tae;Han, Dong Wook;Schoeler, Hans R.
受精卵和早期卵裂球在放入子宫后有可能发育成胎儿，它们是全能的。胚胎中的那些细胞可以产生生物体的所有细胞类型，但不能产生生物体本身，它们是多能的。胚胎干细胞（ES）、胚胎癌细胞（EC）和胚胎生殖细胞（EG）是来自不同胚胎阶段的强大体外人工产物，具有多能性。全能和多能细胞有可能极大地造福于生物研究和医学。一个强大的特征是体细胞的遗传程序可以转化为全能或多能细胞的遗传程序，如核转移或细胞融合实验所示。在通过细胞融合的重编程期间，获得多能细胞的各种特征。这些包括相应多能融合配偶体的典型形态、特定的表观遗传状态、特定的基因谱、体细胞融合配偶体中表达的组织特异性基因的失活以及多能细胞的发育和分化潜能。在这篇综述中，我们将讨论什么是已知的细胞融合介导的重编程过程和融合诱导的重编程治疗应用的潜在用途。
Fertilized eggs and early blastomeres, that have the potential to develop to fetuses when placed into a uterus, are totipotent. Those cells in the embryo, that can give rise to all cell types of an organism, but not to an organism itself, are pluripotent. Embryonic stem (ES), embryonic carcinoma (EC), and embryonic germ (EG) cells are powerful in vitro artifacts derived from different embryonic stages and are pluripotent. Totipotent and pluripotent cells have the potential to greatly benefit biological research and medicine. One powerful feature is that the genetic program of somatic cells can be converted into that of totipotent or pluripotent cells, as shown by nuclear transfer or cell fusion experiments. During reprogramming by cell fusion various features of pluripotent cells are acquired. These include the typical morphology of the respective pluripotent fusion partner, a specific epigenetic state, a specific gene profile, inactivation of tissue-specific genes expressed in the somatic fusion partner, and the developmental as well as differentiation potential of pluripotent cells. In this review, we will discuss what is known about the reprogramming process mediated by cell fusion and the potential use of fusion induced reprogramming for therapeutic applications.