Complete amino acid sequence of chicken cartilage link protein deduced from cDNA clones.
Complete amino acid sequence of chicken cartilage link protein deduced from cDNA clones.
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从 cDNA 克隆推导出鸡软骨连接蛋白的完整氨基酸序列。
DOI：
10.1073/pnas.83.11.3766
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发表时间：
1986
影响因子：
11.1
通讯作者：
Goetinck,PF
中科院分区：
文献类型：
--
作者：
Deák,F;Kiss,I;Sparks,KJ;Argraves,WS;Hampikian,G;Goetinck,PF
分离编码鸡软骨连接蛋白的cDNA克隆并测序。成熟连接蛋白和预测信号肽的整个核心多肽的DNA序列由1065个核苷酸组成。推导的初级翻译产物（355个氨基酸）的分子量为40.7 kDa;成熟连接蛋白核心多肽（340个氨基酸）的计算分子量为39.06 kDa。DNA序列含有两个串联排列的重复序列，其可编码参与与透明质酸结合的连接蛋白的重复功能结构域。鸡连接蛋白的mRNA是6.0、5.8和3.0个内切酶对，RNA种类大小之间的差异在于3'非翻译区。
cDNA clones coding for chicken cartilage link protein were isolated and sequenced. The DNA sequence for the entire core polypeptide of the mature link protein and the predicted signal peptide consists of 1065 nucleotides. The deduced primary translation product (355 amino acids) has a molecular mass of 40.7 kDa; the calculated molecular mass of the mature link protein core polypeptide (340 amino acids) is 39.06 kDa. The DNA sequence contains two tandemly arranged repeat sequences that may code for repeated functional domains of link protein involved in binding to hyaluronic acid. The mRNAs for chicken link protein are 6.0, 5.8, and 3.0 kilobase pairs, and the difference between the sizes of the RNA species lies in the 3' untranslated region.