Comprehensive analysis of the polygalacturonase and pectin methylesterase genes in Brassica rapa shed light on their different evolutionary patterns.
Comprehensive analysis of the polygalacturonase and pectin methylesterase genes in Brassica rapa shed light on their different evolutionary patterns.
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油菜多聚半乳糖醛酸酶和果胶甲酯酶基因的综合分析揭示了它们不同的进化模式
DOI：
10.1038/srep25107
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发表时间：
2016-04-26
影响因子：
4.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Duan W;Huang Z;Song X;Liu T;Liu H;Hou X;Li Y
果胶是植物初生细胞壁中的基本多糖。多聚半乳糖醛酸酶（PGs）和果胶甲基酯酶（PMEs）是果胶重构和分解网络的主要组成部分，在植物发育的许多阶段参与细胞分离过程。对植物中这些基因的全面研究可以揭示其结构发育的进化模式。本研究对芜菁和其他8种植物的PG和PME进行了全基因组注释、分子进化和基因表达分析。在B中共鉴定出100个PG和110个PME。它们主要从12-18 MYA分化，PMEs比PGs保留更多。与8种植物中的305个PG和348个PME一起，发现了两种不同的扩展或进化类型：裸子植物和被子植物分裂后，出现了一个新的A类PG分支，导致PG迅速扩展;综合分析PME基因之间的pro结构域，发现proPME中存在或缺失pro结构域。此外，PG和PME表现出不同的表达模式。这些发现将为基因家族的功能分化和保守性提供新的见解，并为分子进化分析提供更多的支持。
Pectins are fundamental polysaccharides in the plant primary cell wall. Polygalacturonases (PGs) and pectin methylesterases (PMEs), major components of the pectin remodeling and disassembly network, are involved in cell separation processes during many stages of plant development. A comprehensive study of these genes in plants could shed light on the evolution patterns of their structural development. In this study, we conducted whole-genome annotation, molecular evolution and gene expression analyses of PGs and PMEs in Brassica rapa and 8 other plant species. A total of 100 PGs and 110 PMEs were identified in B. rapa; they primarily diverged from 12–18 MYA and PMEs were retained more than PGs. Along with another 305 PGs and 348 PMEs in the 8 species, two different expansion or evolution types were discovered: a new branch of class A PGs appeared after the split of gymnosperms and angiosperms, which led to the rapid expansion of PGs; the pro domain was obtained or lost in the proPMEs through comprehensive analyses among PME genes. In addition, the PGs and PMEs exhibit diverged expression patterns. These findings will lead to novel insight regarding functional divergence and conservation in the gene families and provide more support for molecular evolution analyses.