Pathway engineering for phenolic acid accumulations in Salvia miltiorrhiza by combinational genetic manipulation
Pathway engineering for phenolic acid accumulations in Salvia miltiorrhiza by combinational genetic manipulation
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DOI：
10.1016/j.ymben.2013.10.009
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发表时间：
2014-01-01
影响因子：
8.4
通讯作者：
Wang, Zhe-Zhi
中科院分区：
文献类型：
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作者：
Zhang, Yuan;Yan, Ya-Ping;Wang, Zhe-Zhi
为了生产用于医疗和商业目的的有益酚酸，研究人员感兴趣的是改善由丹参产生的通常低水平的丹参酚酸B（Sal B）。在这里，我们提出了一个战略的组合遗传操作，以丰富可用于Sal B生物合成的前体。这种方法，涉及木质素途径，需要同时，异位表达的拟南芥花青素色素1转录因子（AtPAP 1）加上共抑制两个内源性的关键酶基因：肉桂酰辅酶A还原酶（SmCCR）和愈伤组织酸O-甲基转移酶（SmCOMT）。与未转化的对照相比，我们实现了更大的Sal B积累（高达3倍）沿着降低的木质素浓度。这种高Sal B表型在营养生长期间在根中是稳定的，并且与相应植物提取物的抗氧化能力增加密切相关。虽然表型没有明显的外在变化，但我们通过转录组和代谢组分析的综合分析来表征分子表型。我们的研究结果表明，酚途径扰动对碳水化合物代谢，呼吸，光呼吸和应激反应的深远影响。这份报告是第一个描述生产有价值的最终产品，通过组合遗传操作在S。丹参属植物。我们的策略将是有效的努力代谢工程多分支途径，如苯丙素途径，在经济上重要的药用植物。(C)2013年国际代谢工程学会。爱思唯尔公司出版版权所有© 2016
To produce beneficial phenolic acids for medical and commercial purposes, researchers are interested in improving the normally low levels of salvianolic acid B (Sal B) produced by Salvia miltiorrhiza. Here, we present a strategy of combinational genetic manipulation to enrich the precursors available for Sal B biosynthesis. This approach, involving the lignin pathway, requires simultaneous, ectopic expression of an Arabidopsis Production of Anthocyanin Pigment 1 transcription factor (AtPAP1) plus co-suppression of two endogenous, key enzyme genes: cinnamoyl-CoA reductase (SmCCR) and calleic acid O-methyltransferase (SmCOMT). Compared with the untransformed control, we achieved a greater accumulation of Sal B (up to 3-fold higher) along with a reduced lignin concentration. This high-Sal B phenotype was stable in roots during vegetative growth and was closely correlated with increased antioxidant capacity for the corresponding plant extracts. Although no outward change in phenotype was apparent, we characterized the molecular phenotype through integrated analysis of transcriptome and metabolome profiling. Our results demonstrated the far-reaching consequences of phenolic pathway perturbations on carbohydrate metabolism, respiration, photo-respiration, and stress responses. This report is the first to describe the production of valuable end products through combinational genetic manipulation in S. miltiorrhiza plants. Our strategy will be effective in efforts to metabolically engineer multi-branch pathway(s), such as the phenylpropanoid pathway, in economically significant medicinal plants. (C) 2013 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved,